Sculpting Dynamic Amphiphilic Structures
Lead Research Organisation:
Imperial College London
Department Name: Chemistry
Abstract
Biomembranes lie at the heart of most biological function, and lipid membranes are increasingly finding a wide range of novel applications in biotechnology and nanomedicine. Such self-assembled amphiphilic interfaces can adopt an astonishing range of complex shapes and liquid-crystalline structures ordered in 1, 2 or 3 dimensions, over length scales stretching from 2 - 3 nanometres, to microns. Gaining an understanding at a molecular level of how interface structure, ordering, dynamics and micromechanics depend upon chemical structure and composition, and thermodynamic variables such as temperature, hydration, and pressure, is the key to learning how we can manipulate such self-assembled soft interfaces to create novel and useful structures and new technologies, and this is the main aim of this Programme.
We have identified three key underpinning basic science challenges: 1) asymmetry; 2) patterning; 3) curvature, long-range organisation and symmetry. There are four main aspects underlying these challenges which we consider are of crucial importance: i) compositional asymmetry and dynamics of amphiphile flip-flop across bilayers; ii) lateral segregation, line tension and microdomain formation; iii) membrane curvature and curvature elasticity; iv) charge and dipolar interactions between lipid headgroups. Furthermore, there is a complicated coupling between all of these four aspects, and this is where we will focus much of our attention.
We have assembled a team of five leading UK University research groups, spanning Chemistry, Physics and Biophysics. The groups have complementary expertise covering laboratory-based and synchrotron time-resolved X-ray diffraction, neutron scattering, solid-state nuclear magnetic resonance, calorimetry, biomolecular force microscopy, Langmuir trough and microfluidics technologies, linear and non-linear spectroscopies, atomic force microscopy, spectroscopic and optical imaging, optical tweezers, microrheology, and theory. These approaches will be used to attack different inter-related aspects of the three key basic science challenges. We will ensure an efficient translation and synthesis of all of the findings, by a tightly- regulated management structure, and by regular meetings and staff exchanges between the five research groups.
Building on the engineering rules and technologies developed previously in the programme, we will integrate the earlier work to develop lipid structures into active lipid systems such as: self-encapsulated droplet interface bilayer networks in water; patterned asymmetric vesicles of defined size: coupling microfluidics with smart droplet microtools; phospholipid phases and vesicles in thermal gradients.
We will then use this knowledge to develop three demonstration systems:
i) Artificial Organelles. The development of artificial organelle machines which mimic some of the remarkable functions and properties of biology will lead to new approaches for personalized healthcare.
ii) Rapid drug-membrane binding screen. A compartmentalised, rapid drug screening device will allow parallel measurements of drug interactions with a number of artificial plasma membrane mimics (PMMs) formed by an array of parallel droplet interface bilayer or vesicle networks.
iii) In-Cubo Crystallization of Large Membrane Proteins. Learning how to swell lipid cubic phases will unlock our ability to construct cubic scaffolds with unit cell dimensions of the order of tens or hundreds of nanometres, allowing incorporation of large membrane proteins (>50kD), which are major drug targets for the pharmaceutical industry.
Further biological and biotechnological applications will be developed during the course of the Programme by the current Investigators and a wider group of industrial and academic collaborators, who will be brought into the Programme as appropriate.
We have identified three key underpinning basic science challenges: 1) asymmetry; 2) patterning; 3) curvature, long-range organisation and symmetry. There are four main aspects underlying these challenges which we consider are of crucial importance: i) compositional asymmetry and dynamics of amphiphile flip-flop across bilayers; ii) lateral segregation, line tension and microdomain formation; iii) membrane curvature and curvature elasticity; iv) charge and dipolar interactions between lipid headgroups. Furthermore, there is a complicated coupling between all of these four aspects, and this is where we will focus much of our attention.
We have assembled a team of five leading UK University research groups, spanning Chemistry, Physics and Biophysics. The groups have complementary expertise covering laboratory-based and synchrotron time-resolved X-ray diffraction, neutron scattering, solid-state nuclear magnetic resonance, calorimetry, biomolecular force microscopy, Langmuir trough and microfluidics technologies, linear and non-linear spectroscopies, atomic force microscopy, spectroscopic and optical imaging, optical tweezers, microrheology, and theory. These approaches will be used to attack different inter-related aspects of the three key basic science challenges. We will ensure an efficient translation and synthesis of all of the findings, by a tightly- regulated management structure, and by regular meetings and staff exchanges between the five research groups.
Building on the engineering rules and technologies developed previously in the programme, we will integrate the earlier work to develop lipid structures into active lipid systems such as: self-encapsulated droplet interface bilayer networks in water; patterned asymmetric vesicles of defined size: coupling microfluidics with smart droplet microtools; phospholipid phases and vesicles in thermal gradients.
We will then use this knowledge to develop three demonstration systems:
i) Artificial Organelles. The development of artificial organelle machines which mimic some of the remarkable functions and properties of biology will lead to new approaches for personalized healthcare.
ii) Rapid drug-membrane binding screen. A compartmentalised, rapid drug screening device will allow parallel measurements of drug interactions with a number of artificial plasma membrane mimics (PMMs) formed by an array of parallel droplet interface bilayer or vesicle networks.
iii) In-Cubo Crystallization of Large Membrane Proteins. Learning how to swell lipid cubic phases will unlock our ability to construct cubic scaffolds with unit cell dimensions of the order of tens or hundreds of nanometres, allowing incorporation of large membrane proteins (>50kD), which are major drug targets for the pharmaceutical industry.
Further biological and biotechnological applications will be developed during the course of the Programme by the current Investigators and a wider group of industrial and academic collaborators, who will be brought into the Programme as appropriate.
Planned Impact
Our ambition within this Programme grant is to exploit the emerging field of molecular membrane engineering, by designing and constructing new biologically-inspired parts, devices and systems with user-defined levels of asymmetry, patterning and curvature. This will revolutionise the design and fabrication of smart, soft materials based on the lipid bilayer as the basic structural motif, with morphologies spanning the nano to micron lengthscale, and dynamics controlled over a wide range of timescales. In the longer term this will lead to a paradigm shift in areas such as nanomedicine, bioelectronics, biological computing devices and synthetic cells.
To achieve this we will bring together five leading UK research groups to investigate experimentally and theoretically the rules underpinning the self-assembly, ordering and dynamics of amphiphilic systems.
This knowledge will then be exploited:
i) to develop quantitative engineering rules for construction of 2-D and 3-D membrane-based structures with targeted asymmetry, patterning and curvature, focussing initially on purely lipid-based systems;
ii) to design self-assembled, fluid, amphiphilic systems with useful functional properties;
iii) to develop demonstrate applications, such as artificial organelles, high-throughput screens for drug discovery, and swollen cubic phases for membrane protein crystallisation.
We plan to invite companies with interests in these areas to participate by funding Industrial PhD Students from year 3 onwards to develop the applications, and to help embed the knowledge gained within an industrial context. We will spend £10k from our own resources on direct commercialisation activities, led by the ICB and the PM. They, together with the consortium, will:
a) network with relevant TSB Knowledge Transfer Networks and other industrial networks that may have an interest in these technologies;
b) develop Outline Concept Specifications documents as a starting point for discussions with potential users and developers of the technologies;
c) form an Industry Consortium. In the final year of the project, an Industrial Showcase event will be held at Durham.
As this project extends the current state-of-the art we expect to generate intellectual property with significant commercial value, and will protect it via established technology transfer organisations who will cover the patenting costs. A consortium agreement will govern the IP generated, and the Advisory Board will be bound by a confidentiality agreement.
We will organise a Workshop in year 3, with invitees from both academia and industry, and an International Meeting in year 5. We will embed public engagement activities within the Programme, such as the Royal Society's Summer Science Exhibition. We will highlight the field of self-assembled complex soft materials by making conference lectures freely available on iTunesU /YouTube and by a radio broadcast produced as part of the Imperial College Institute of Chemical Biology DTC Science Communication course. This will complement dissemination via websites, meetings, conferences and journal publications.
We expect the more junior Investigators and some of the post-doctoral researchers to become research leaders, either in industry or academia, within 5-10 years.
To achieve this we will bring together five leading UK research groups to investigate experimentally and theoretically the rules underpinning the self-assembly, ordering and dynamics of amphiphilic systems.
This knowledge will then be exploited:
i) to develop quantitative engineering rules for construction of 2-D and 3-D membrane-based structures with targeted asymmetry, patterning and curvature, focussing initially on purely lipid-based systems;
ii) to design self-assembled, fluid, amphiphilic systems with useful functional properties;
iii) to develop demonstrate applications, such as artificial organelles, high-throughput screens for drug discovery, and swollen cubic phases for membrane protein crystallisation.
We plan to invite companies with interests in these areas to participate by funding Industrial PhD Students from year 3 onwards to develop the applications, and to help embed the knowledge gained within an industrial context. We will spend £10k from our own resources on direct commercialisation activities, led by the ICB and the PM. They, together with the consortium, will:
a) network with relevant TSB Knowledge Transfer Networks and other industrial networks that may have an interest in these technologies;
b) develop Outline Concept Specifications documents as a starting point for discussions with potential users and developers of the technologies;
c) form an Industry Consortium. In the final year of the project, an Industrial Showcase event will be held at Durham.
As this project extends the current state-of-the art we expect to generate intellectual property with significant commercial value, and will protect it via established technology transfer organisations who will cover the patenting costs. A consortium agreement will govern the IP generated, and the Advisory Board will be bound by a confidentiality agreement.
We will organise a Workshop in year 3, with invitees from both academia and industry, and an International Meeting in year 5. We will embed public engagement activities within the Programme, such as the Royal Society's Summer Science Exhibition. We will highlight the field of self-assembled complex soft materials by making conference lectures freely available on iTunesU /YouTube and by a radio broadcast produced as part of the Imperial College Institute of Chemical Biology DTC Science Communication course. This will complement dissemination via websites, meetings, conferences and journal publications.
We expect the more junior Investigators and some of the post-doctoral researchers to become research leaders, either in industry or academia, within 5-10 years.
Publications
Shimobayashi S
(2015)
Direct measurement of DNA-mediated adhesion between lipid bilayers
Karamdad K
(2015)
Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method.
in Lab on a chip
Zhang Y
(2015)
A coarse-grained molecular dynamics investigation of the phase behavior of DPPC/cholesterol mixtures.
in Chemistry and physics of lipids
Carreras P
(2015)
A microfluidic platform for size-dependent generation of droplet interface bilayer networks on rails
in Biomicrofluidics
Richens JL
(2015)
Optimisation of protein microarray techniques for analysis of the plasma proteome: minimisation of non-specific binding interactions.
in International immunopharmacology
Barriga H
(2015)
Temperature and pressure tuneable swollen bicontinuous cubic phases approaching nature's length scales
in Soft Matter
Elani Y
(2015)
Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors.
in Physical chemistry chemical physics : PCCP
Richens JL
(2015)
The interactions of squalene, alkanes and other mineral oils with model membranes; effects on membrane heterogeneity and function.
in Journal of colloid and interface science
Williamson J
(2015)
Kinetics of symmetry and asymmetry in a phase-separating bilayer membrane
Elani Y
(2015)
Measurements of the effect of membrane asymmetry on the mechanical properties of lipid bilayers.
in Chemical communications (Cambridge, England)
Description | We have carried out measurements - some for the first time - of: a. Effect of membrane asymmetry on bilayer rigidity; b. Effect of hydrostatic pressure on the mechanical properties of lipid bilayers; c. Lifetime of membrane asymmetry using fluctuation analysis/SFG; d. Interleaflet registration in phase-separated bilayers; e. Coupling mobile ligands to soft membranes; f. Soft pinning of liquid domains on caps; g. Diffusion in lipid membranes; h. Critical fluctuations in lipid mixtures by AFM. We have developed: a. Highly swollen cubic phases with dimensions comparable with those seen in Nature; b. Vesicles with user defined size, asymmetry, cargo and biomolecule content; c. Compartmentalised vesicles; d. Extended 2-D/3-D droplet interface bilayer networks; e. Compartmentalised extended lifetime multisomes; f. Membrane encapsulated nanoparticles. In terms of applications, we have developed: a. Artificial cells capable of acting as micro-reactors, as well as performing in-situ drug synthesis, environmental sensing and smart delivery. The complexity of these systems is now such that we have transplanted spatially separated enzymatic pathways into artificial protocells; b. High-throughput screens for drug discovery; c. Studies of membrane translocation profiles for agrichemicals (Syngenta), PROTAC molecules (GSK plc) and hair additives (P&G) using droplet interface bilayer networks. |
Exploitation Route | By bringing together five world leading UK research groups, this Programme grant has pioneered a series of breakthroughs in the emerging field of molecular membrane engineering that have already supported 40 publications, 38 high profile international conferences, 33 oral and poster presentations, £20M+ in follow-on funding and 3 industrial collaborations. The successful development of quantitative engineering rules for the construction of 2-D and 3-D membrane-based structures with user defined levels of asymmetry, patterning and curvature has enabled us to extend the aims and ambitions of the project beyond proof of concept applications to include direct commercial exploitation. New collaborations with leading technology companies in the pharmaceutical (GSK plc), agri-tech (Syngenta) and personal care sectors (P&G) should led to the translation of the innovations from the Programme grant into the industrial sector. |
Sectors | Agriculture, Food and Drink,Chemicals,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | This Programme grant has had a huge impact in the field. It has led to over 150 refereed publications. The Investigators have given numerous Invited Lectures at international conferences as well as many Seminars and outreach talks to a more general audience. They have also organised key conferences in the field, such as the Telluride Workshop on Physics and Chemistry of Lipid Membranes, 4 - 8 July 2016, USA; an RSC Faraday Discussion on Peptide - Membrane Interactions, 8 - 10 Sept 2021 (held online); a Royal Society Discussion Meeting on Cell Mimicry, 7 - 8 Nov 2022, Royal Society, London. The Imperial team have been recognized by the Imperial College President's Award for Outstanding Research Team in 2015, and the Rita and John Cornforth Award of the RSC in 2017. This latter award was linked to a series of Award Lectures, given by the team at the Universities of Brighton, Lincoln, Southampton, Queens University Belfast, and UCL. Members of the team have sat on a range of Review Panels (EPSRC, ESRF, Diamond). There are numerous examples of Outreach, for example schools lectures, and most recently the Imperial team will have a stall on 'Artificial Cells' at the Great Exhibition Road Festival 2023, from 17-18 June 2023. |
First Year Of Impact | 2015 |
Sector | Agriculture, Food and Drink,Chemicals,Education,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural,Societal,Policy & public services |
Description | Biophysical Sciences Institute Summer undergraduate research studentship |
Amount | £2,000 (GBP) |
Organisation | Durham University |
Department | Biophysical Sciences Institute |
Sector | Academic/University |
Country | United Kingdom |
Start | 07/2014 |
End | 09/2014 |
Description | Leverhulme Doctoral Scholarship Programme |
Amount | £1,000,000 (GBP) |
Organisation | The Leverhulme Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 10/2018 |
End | 09/2021 |
Title | 0 |
Description | |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | Yes |
Title | Dataset associated with "Nanoscale Substrate Roughness Hinders Domain Formation in Supported Lipid Bilayers" |
Description | Supported Lipid Bilayers (SLBs) are model membranes formed at solid substrate surfaces. This architecture renders the membrane experimentally accessible to surface sensitive techniques used to study their properties, including Atomic Force Microscopy (AFM), optical fluorescence microscopy, Quartz Crystal Microbalance (QCM) and X-Ray/Neutron Reflectometry, and allows integration with technology for potential biotechnological applications such as drug screening devices. The experimental technique often dictates substrate choice or treatment, and it is anecdotally recognised that certain substrates are suitable for the particular experiment, but the exact influence of the substrate has not been comprehensively investigated. Here, we study the behavior of a simple model bilayer, phase separating on a variety of commonly used substrates, including glass, mica, silicon and quartz, with drastically different results. The distinct micron scale domains observed on mica, identical to those seen in free-floating Giant Unilamellar Vesicles (GUVs), are reduced to nanometer scale domains on glass and quartz. The mechanism for the arrest of domain formation is investigated, and the most likely candidate is nanoscale surface roughness, acting as a drag on the hydrodynamic motion of small domains during phase separation. Evidence was found that the physico-chemical properties of the surface have a mediating effect, most likely due to changes in the lubricating interstitial water layer between surface and bilayer. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | Yes |
URL | http://archive.researchdata.leeds.ac.uk/796/ |
Title | Research Data Supporting "A Robust Liposomal Platform For Direct Colorimetric Detection Of Sphingomyelinase Enzyme And Inhibitors" |
Description | Raw research data supporting the publication: Holme, M. N. et al., ACS Nano, 2018, DOI: 10.1021/acsnano.8b03308. |
Type Of Material | Database/Collection of data |
Year Produced | 2018 |
Provided To Others? | Yes |
Description | Jonas Camillus Jeppesen - visiting international PhD student from the lab of Adam Simon Cohenson in Odense, Denmark |
Organisation | University of Southern Denmark |
Country | Denmark |
Sector | Academic/University |
PI Contribution | Access to high-speed AFM, training, and techniques to study membrane dynamics with temperature control. Collaborator brought new samples and system well studied in their lab. The project was to study the early stages of domain nucleation in gel-phase binary lipid mixtures. |
Collaborator Contribution | None |
Impact | None-to-date, work still in progress |
Start Year | 2015 |
Description | CECAM Workshop |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Workshop entitled "Multi scale approaches to nano-structured soft matter: from amphiphiles to block copolymers". |
Year(s) Of Engagement Activity | 2018 |
Description | Co-organiser, Royal Society Discussion Meeting on 'Cell mimicry: bottom-up engineering of life' |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Co-organiser, Royal Society Discussion Meeting on 'Cell mimicry: bottom-up engineering of life', 21st - 22nd September 2020, Royal Society London. |
Year(s) Of Engagement Activity | 2020 |
Description | Crossing BioMembranes NET Conference, September 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | CBM (Crossing BioMembranes) NET Conference, September 2015. |
Year(s) Of Engagement Activity | 2015 |
Description | Deputy Chair of Scientific Committee |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Deputy Chair of Scientific Committee: Faraday Discussion on Peptide - Membrane Interactions, Royal Society of Chemistry, London, 8th - 10th September 2021. Held Online. |
Year(s) Of Engagement Activity | 2021 |
Description | Deputy Chair of Scientific Committee, Faraday Discussion |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Deputy Chair of Scientific Committee, Faraday Discussion on Peptide - Membrane Interactions, Royal Society of Chemistry, London, 7th - 9th September 2020. |
Year(s) Of Engagement Activity | 2020 |
Description | Drug Disposition Symposium, Stevenage, June 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Drug Disposition Symposium, Stevenage, June 2015 |
Year(s) Of Engagement Activity | 2015 |
Description | Faraday Discussion on Peptide - Membrane Interactions |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Faraday Discussion on Peptide - Membrane Interactions, RSC, London, 8 - 10 Sept 2021 |
Year(s) Of Engagement Activity | 2021 |
Description | Graduate school teaching, ICTP Trieste, July 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Pietro Cicuta was involved in a hands-on graduate school in Trieste, Italy. This event increased discussion and knowledge transfer Close interactions with graduate students and young faculty from developing countries helped to introduce new tools and techniques to the group, encouraging the development of scientific leaders in less developed countries |
Year(s) Of Engagement Activity | 2014 |
Description | Halim Kusumaatmaja Invited Conference talk - 115th Statistical Mechanics Conference, Rutgers University, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Surveying Free Energy Landscapes: Applications to Continuum Soft Matter Systems, Rutgers University, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Invited Conference talk - : Fundamentals and Applications of Lipid Membrane Biophysics, London, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Continuum Theory of Lipid Membranes, London, UK |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Invited Conference talk - Partial Order: Mathematics, Simulations and Applications, Institute for Pure and Applied Mathematics, UCLA, Los Angeles, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Surveying Free Energy Landscapes: Applications to Continuum Soft Matter Systems, UCLA, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Department of Mathematics, Pennsylvania State University, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Wetting on Lipid Membranes, Pennsylvania, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Department of Mathematics, University of York, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Membrane Phase Separation, York, UK |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Seminar talk - Department of Physics, University of Oxford, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Oxford, UK |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Institut Lumière Matière |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Lyon, France |
Year(s) Of Engagement Activity | 2017 |
Description | Halim Kusumaatmaja Seminar talk - State Key Laboratory of Polymer Physics and Chemistry, Changchun, China |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Curvature-Induced Phase Separation on Lipid Membranes, Changchun, China |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Seminar talk - University of Rome Tor Vergata, Rome, Italy |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Rome, Italy |
Year(s) Of Engagement Activity | 2015 |
Description | IC-ANMBES 2021 Conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Supporters |
Results and Impact | IC-ANMBES 2021 Conference, Brasov, Romania: 2 - 4 June 2021. |
Year(s) Of Engagement Activity | 2021 |
Description | International Microfluidics Conference, London, October 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Microfluidics Conference, held in London over 2 days 20/21 October 2015. |
Year(s) Of Engagement Activity | 2015 |
Description | Invited Lecture |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 7th European Joint Theoretical / Experimental Meeting on Membranes, Graz, 7th - 9th April 2021. Lecture given Online. |
Year(s) Of Engagement Activity | 2021 |
Description | Invited Lecture, Brasov, Romania |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, IC-ANMBES 2020 conference, Brasov, Romania, 16th - 18th June 2020 |
Year(s) Of Engagement Activity | 2020 |
Description | Invited Lecture, Daejeon, March 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja invited to present a talk at the British Council workshop on soft matter Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited Lecture, El Escorial, Spain |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Biophysics Summer School, El Escorial, Spain, 18th - 20th July 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Lecture, Graz |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, 7th European Joint Theoretical / Experimental Meeting on Membranes, Graz, 6th - 8th May 2020. |
Year(s) Of Engagement Activity | 2020 |
Description | Invited Lecture, Leeds |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Workshop on Lyotropic Liquid Crystals (Satellite to Annual British Liquid Crystal Conference), Leeds, 15th - 17th April 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Lecture, Lund University |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Lund University, LINX Mini-Symposium, 14th March 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Plenary Lecture - Dynamics in Biological AFM, AFM Conference and Workshop, |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Requimte AFM Conference and Workshop, University of Porto, Portugal. |
Year(s) Of Engagement Activity | 2016 |
Description | Invited lecture and workshop, Julich, October 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Pietro Cicuta was invited to present a student lecture and workshop on the subject of particles interactions with membranes. The event engaged and sparked discussion and encouraged future collaborative activity Engaged and sparked discussion, encouraged future collaborative activity |
Year(s) Of Engagement Activity | 2014 |
URL | http://www.fz-juelich.de/ics/ics-2/EN/Leistungen/ConferencesAndWorkshops/Particles@Membranes/Venue/_... |
Description | Invited lecture, Austria, August 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell invited to present a talk on Critical Membranes at the Bio-Nano summer school in Austria on the 14th August 2013. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Austria, August 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presented a talk entitled 'Membrane Biophysics' at a Bio-Nano summer school in Austria in August 2014 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Coventry, September 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Simon Connell invited to present a talk on Fast Scan AFM dynamics in lipid membranes at the Bruker Nanoelectrical and Chemical workshop in Coventry on the 25th September 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Durham, August 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja was invited to present a talk at the 2014 Energy Landscapes symposium and workshop hosted at Durham University. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Durham, December 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | John Sanderson invited to a Cafe Scientifique talk about the chemistry of biological membranes. The talk increased awareness, engagement and discussion around this topic. Knowledge transfer, networking and potential for future collaborative activity |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Goettingen, November 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja was invited to present a talk at the Max Plank Institute for Dynamics and and Self Organisation in Goettingen in November 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, London, September 2012 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell invited to present a talk on Critical fluctuations in Lipid Membranes at Faraday Discussions 160, on 11th September 2012 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2012 |
Description | Invited lecture, Lyon, September 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja invited to present a talk on the influence of interfacial interactions on structural and rheological properties of confined liquids in Lyon in September 2013. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Toronto, September 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | SImon Connell invited to present a talk on fast scan dynamics in lipid membranes at SPM on SPM in Toronto on the 5th September 2014 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, York, March 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | John Seddon invited present a lecture at the Bruker Poster Prize Day at the Unviersity of York on the 20th March 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited workshop contributor, London, February 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | John Seddon was an invited participant at an EPSRC Physical Sciences leaders workshop at Imperial College London in February 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | John Sanderson, Chair - Membrane Peptides and Toxins |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Seminars: Membrane Peptides and Toxins (Session Chair, Biophysical Society Annual Meeting, New Orleans, February 11 - 15, 2017. |
Year(s) Of Engagement Activity | 2017 |
Description | Meeting with the Chair of RSC Faraday Standing Committee on Conferences |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Meetings with the Chair of RSC Faraday Standing Committee on Conferences |
Year(s) Of Engagement Activity | 2017,2018,2019,2020 |
Description | Meeting with the Co-Chair, EPSRC Physical Sciences Grants Meeting |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | , EPSRC Physical Sciences Grants Meeting (5th December 2018); |
Year(s) Of Engagement Activity | 2018 |
Description | Meeting with the Secretary, European Biophysics Societies' Association |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Meeting with the Secretary, European Biophysics Societies' Association |
Year(s) Of Engagement Activity | 2013,2014,2015,2016,2017,2018,2019,2020 |
Description | Membranes Conference - EJTEMM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | EJTEMM Membranes Conference, Graz, 7-9 April 2021 |
Year(s) Of Engagement Activity | 2021 |
Description | One Life: Science At The Interface, September 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | One Life: Science At The Interface, September 2015 |
Year(s) Of Engagement Activity | 2015 |
Description | Oral presentation, Lancaster, July 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Simon Connell presented a lecture at the EPSRC Physics of Life summer school at Lancaster University on 18th July 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Oral presentation, Leeds, June 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presented a talk at the Royal Microscopy Society in Leeds on 26th June 2013. The talk encouraged further questions and debate Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration. |
Year(s) Of Engagement Activity | 2013 |
Description | Oral presentation, London, September 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Nicola McCarthy was invited to present a talk at the European High Pressure Research Group international conference in London. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Oral presentation, Manchester, October 2012 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presenteda talk on surface science of biologically important interfaces on the 18th October 2012. The event initiated further questions and discussion among the audience. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration. |
Year(s) Of Engagement Activity | 2014 |
Description | Plenary Lecture, Université Paris Descartes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Plenary Lecture, ECOF 16th European Conference on Organized Films, Université Paris Descartes, 9th - 12th July 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Poster: Drug Lipidation in Membranes (full author list: Hannah M. Britt, Vian S. Ismail, Jackie A. Mosely and John M. Sanderson) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster: Drug Lipidation in Membranes, Biophysical Society Annual Meeting, New Orleans, February 11 - 15, 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Poster: Drug Lipidation in Membranes (full author list: Hannah M. Britt, Vian S. Ismail, Jackie A. Mosely and John M. Sanderson) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster: Drug Lipidation in Membranes, Biophysical Society Annual Meeting, Los Angeles, February 27-March 2, 2016 |
Year(s) Of Engagement Activity | 2016 |
Description | Remote Review Panel |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Remote Review Panel: ESRF, Grenoble, Beamline ID02; 2 - 3 November 2021. |
Year(s) Of Engagement Activity | 2021 |
Description | Royal Society Discussion Meeting on Cell Mimicry |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Royal Society Discussion Meeting on Cell Mimicry, 7 - 8 Nov 2022, Royal Society London |
Year(s) Of Engagement Activity | 2022 |
Description | Scientific Committee and Session Chair |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Scientific Committee and Session Chair: EBSA European Biophysics Congress, Vienna, 24 - 28 July 2021. Hybrid meeting. |
Year(s) Of Engagement Activity | 2021 |
Description | Seminar at Edinburgh University, Chemistry Department |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Seminar at the Dept of Chemistry, University of Edinburgh - 6 November 2019 |
Year(s) Of Engagement Activity | 2019 |
Description | Simon Connell Conference talk - Critical Dynamics in supported membranes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 8th-10th December 2016 "Critical Dynamics in supported membranes", Biological Interfaces - Interfacing with Biology, University of Leeds (International Conference) |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Fast Scan AFM of lipid dynamics |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 13-15th December 2016, "Fast Scan AFM of lipid dynamics" UK SPM Conference, Department of Materials Science and Metallurgy, Cambridge University. |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Fluctuating Lipid Nanodomains near Critical Transitions |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 4th-8th July 2016 "Fluctuating Lipid Nanodomains near Critical Transitions", Complexity in the Chemistry and Physics of lipid membranes, Telluride TSRC Workshop, Telluride , Colorado, USA. |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Quantifying lipid membrane dynamics using Fast Scan AFM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 26th-30th August 2016 "Quantifying lipid membrane dynamics using Fast Scan AFM", SPM-on-SPM, Changchun, China |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell seminar talk - AFM Studies of Bilayer Dynamics, Structure and Growth in the Three-Phase Region |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 13th February 2015, "AFM Studies of Bilayer Dynamics, Structure and Growth in the Three-Phase Region", Departmental Seminar, Olmsted Group, Georgetown University, USA. |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Critical Fluctuations in Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 27th October 2016 "Critical Fluctuations in Lipid Bilayers", Theoretical Physics Group Seminar, Department of Physics, University of Warwick |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell seminar talk - Lipid Nanodomain Dynamics: Fast AFM. Line tensions, Ising Models and coupling Modes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 8th Feb 2017, "Lipid Nanodomain Dynamics: Fast AFM. Line tensions, Ising Models and coupling Modes", London School of Nanotechnology (LCN) Colloquium. |
Year(s) Of Engagement Activity | 2017 |
Description | Simon Connell seminar talk - Membranes, Microfluidics and Microbubbles: from fundamentals to clinical application |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 13th April 2015 "Membranes, Microfluidics and Microbubbles: from fundamentals to clinical application", Interdisciplinary Computing and Complex BioSystems Research Group, School of Computing Science, University of Newcastle |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 16th February 2015, "Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers", Departmental Seminar, Dufresne Group, Yale University, USA. |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 13th March 2015, "Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers", Departmental Seminar, Ando Group, Kanazawa University, Japan, |
Year(s) Of Engagement Activity | 2015 |
Description | SoftComp Annual meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Presentation in Ancona, Italy at the SoftComp annual meeting - Demixing transitions in Bicontinuous Cubic Phases close to the critical point |
Year(s) Of Engagement Activity | 2016 |
Description | StatPhys 26 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Talk at StatPhys, Lyon, France - Demixing transitions in Bicontinuous Cubic Phases close to the critical point |
Year(s) Of Engagement Activity | 2016 |
Description | Vesicle Carriers Symposium, Manchester, March 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Presented talk at the University of Manchester on Vesicle Carriers. The symposium was spread over 2 days - 4th and 5th March 2016 and was fully attended. |
Year(s) Of Engagement Activity | 2016 |
Description | Zing Computational Chemical Biology Conference, 6-10 August 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Zing Computational Chemical Biology conference, invited delegates from a worldwide audience, held over the 6-10 August 2015. |
Year(s) Of Engagement Activity | 2015 |