Nuclear organisation and erythropoiesis
Lead Research Organisation:
University of Oxford
Department Name: UNLISTED
Abstract
Basic DNA sequence is only a starting block for understanding the complex interplay of factors that regulate the ability of a gene to make the correct protein at the correct time and in the correct cell type. We have detailed maps of a region of chromosome 16 around the alpha globin genes with regulatory features within the sequence and additional (epigenetic) factors which can influence when and where a gene becomes active. DNA coils down within a cell nucleus with several proteins to form chromatin. We now want to look inside individual cells at the way chromatin is organised around the alpha globin genes when they are switched on and off. We would like to understand how this chromatin is arranged within a nucleus and what changes in conformation and condensation may be necessary to allow genes to become active. We will also look at how DNA replicates itself in this region and whether that alters when the alpha globin genes are very active. Understanding these very basic concepts of how genes are regulated is an important foundation to developing new ways to modify gene activity for patients with inherited anaemias.
Technical Summary
The organisation of chromatin within the nucleus has a key role to play in the processes of transcription and replication but the mechanisms governing how chromatin domains are established and altered during the course of development and differentiation remain poorly understood. There is a broad consensus that chromatin is organised in dynamic loops but many questions remain about what may drive their formation, how long they are stable for and how they may alter with the cell cycle. The ? globin gene region is one of the best-characterised segments of the genome and it is an ideal model with which to investigate such issues. Analysis of single cells will be an essential complement to the genome-wide studies of chromatin configurations described in Professors Higgs & Gibbons’ programme. Our ultimate aim is to gain better insight into transcriptional regulation. The questions we plan to address are – What are the kinetics of transcription from the ? globin and nearby genes during erythroid differentiation? What are the concurrent spatial relationships across the ? globin regulatory region? What mechanisms underlie the striking decompaction of chromatin around these heavily transcribed genes? What contribution may replication make to gene regulation and also to misregulation in disease?
People |
ORCID iD |
| Veronica Buckle (Principal Investigator) |
Publications
Babbs C
(2020)
ATR-16 syndrome: mechanisms linking monosomy to phenotype.
in Journal of medical genetics
Badat M
(2023)
Direct correction of haemoglobin E ß-thalassaemia using base editors.
in Nature communications
Bejjani F
(2021)
Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers.
in Nucleic acids research
Brown JM
(2018)
A tissue-specific self-interacting chromatin domain forms independently of enhancer-promoter interactions.
in Nature communications
Brown JM
(2022)
RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure.
in Nature protocols
Chiariello AM
(2020)
A Dynamic Folded Hairpin Conformation Is Associated with a-Globin Activation in Erythroid Cells.
in Cell reports
Di Genua C
(2021)
To bi or not to bi: Acute erythroid leukemias and hematopoietic lineage choice.
in Experimental hematology
Downes DJ
(2023)
Author Correction: Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
in Nature protocols
Related Projects
| Project Reference | Relationship | Related To | Start | End | Award Value |
|---|---|---|---|---|---|
| MC_UU_00016/1 | 31/03/2017 | 30/03/2022 | £3,035,000 | ||
| MC_UU_00016/2 | Transfer | MC_UU_00016/1 | 31/03/2017 | 30/03/2022 | £3,411,000 |
| MC_UU_00016/3 | Transfer | MC_UU_00016/2 | 31/03/2017 | 30/03/2022 | £1,366,000 |
| MC_UU_00016/4 | Transfer | MC_UU_00016/3 | 31/03/2017 | 30/03/2020 | £3,017,000 |
| MC_UU_00016/5 | Transfer | MC_UU_00016/4 | 31/03/2017 | 30/03/2020 | £497,000 |
| MC_UU_00016/6 | Transfer | MC_UU_00016/5 | 31/03/2017 | 30/03/2022 | £2,530,000 |
| MC_UU_00016/7 | Transfer | MC_UU_00016/6 | 31/03/2017 | 30/03/2022 | £2,018,000 |
| MC_UU_00016/8 | Transfer | MC_UU_00016/7 | 31/03/2017 | 30/03/2018 | £1,131,000 |
| MC_UU_00016/9 | Transfer | MC_UU_00016/8 | 31/03/2017 | 30/03/2022 | £2,500,000 |
| MC_UU_00016/10 | Transfer | MC_UU_00016/9 | 31/03/2017 | 30/03/2018 | £1,171,000 |
| MC_UU_00016/11 | Transfer | MC_UU_00016/10 | 31/03/2017 | 30/03/2022 | £1,387,000 |
| MC_UU_00016/12 | Transfer | MC_UU_00016/11 | 31/03/2017 | 30/03/2022 | £446,000 |
| Title | Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin |
| Description | Here we use 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify linked chromatin domains (CDs) composed of irregular ~200-300-nm-wide aggregates of nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin region. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications towards the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin, but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization. |
| Type Of Art | Image |
| Year Produced | 2020 |
| URL | https://idr.openmicroscopy.org/webclient/?show=project-1152 |
| Title | Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin |
| Description | Here we use 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify linked chromatin domains (CDs) composed of irregular ~200-300-nm-wide aggregates of nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin region. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications towards the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin, but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization. |
| Type Of Art | Image |
| Year Produced | 2020 |
| URL | https://idr.openmicroscopy.org/webclient/?show=project-1160 |
| Title | Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin |
| Description | Here we use 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify linked chromatin domains (CDs) composed of irregular ~200-300-nm-wide aggregates of nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin region. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications towards the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin, but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization. |
| Type Of Art | Image |
| Year Produced | 2020 |
| URL | https://idr.openmicroscopy.org/webclient/?show=project-1159 |
| Title | Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin |
| Description | Here we use 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify linked chromatin domains (CDs) composed of irregular ~200-300-nm-wide aggregates of nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin region. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications towards the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin, but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization. |
| Type Of Art | Image |
| Year Produced | 2020 |
| URL | https://idr.openmicroscopy.org/webclient/?show=project-1161 |
| Title | Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin |
| Description | Here we use 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify linked chromatin domains (CDs) composed of irregular ~200-300-nm-wide aggregates of nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin region. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications towards the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin, but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization. |
| Type Of Art | Image |
| Year Produced | 2020 |
| URL | https://idr.openmicroscopy.org/webclient/?show=project-1158 |
| Title | Educational dance 'Cell' |
| Description | The performance CELL explores human cells through intricate choreography. Using giant inflatable nucleus and cells, it is a playful exploration of cell biology and immunology, inspired by life in the lab, the actions of cells and the folding of DNA. The production features a soundtrack by Grammy award-winner Tchad Blake and an inflatable set designed by renowned environmental artist Steve Messam. The installation-like cells come alive with movement to make audiences marvel at the body's power, quirks and hidden landscapes and to allow young people to discover the life-giving world inside their bodies; a universe smaller than a grain of sand. |
| Type Of Art | Performance (Music, Dance, Drama, etc) |
| Year Produced | 2019 |
| Impact | As supporting material, the dance company have produced a comic that is being given to all attendees as well as explanatory videos to help teachers and Q&A sessions, all featuring an avatar of Prof Buckle. |
| URL | https://www.imm.ox.ac.uk/about/news/arts-meets-science-in-dance-exploration-of-cell-biology |
| Description | Dynamics in the regulatory genome. |
| Amount | £162,000 (GBP) |
| Funding ID | 220046/Z/19/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2023 |
| Description | Independent charity reg no: 1108692 |
| Amount | £82,000 (GBP) |
| Organisation | Reuben and Friends |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 08/2017 |
| End | 02/2019 |
| Description | Understanding the basis of Rare Anaemias |
| Amount | £38,342 (GBP) |
| Funding ID | RCF18/033 |
| Organisation | University of Oxford |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 03/2020 |
| Description | Wellcome Discovery Award |
| Amount | £3,738,985 (GBP) |
| Funding ID | 225220/Z/22/Z |
| Organisation | University of Oxford |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2022 |
| End | 09/2027 |
| Title | CSynth |
| Description | Pilot project for the dynamic visualization of 3D nuclear structure |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Developed in collaboration with Stephen Taylor and Goldsmiths university this tool allows for the interaction and interrogation of 3-Dimensional Chromatin structure. The aim being to provide and an intuitive way of humans interacting with complex 3D structure in the nucleus to further our understanding of gene regulation. |
| URL | http://www.csynth.org/ |
| Title | DeepC: predicting 3D genome folding using megabase-scale transfer learning |
| Description | Predicting the impact of noncoding genetic variation requires interpreting it in the context of three-dimensional genome architecture. We have developed deepC, a transfer-learning-based deep neural network that accurately predicts genome folding from megabase-scale DNA sequence. DeepC predicts domain boundaries at high resolution, learns the sequence determinants of genome folding and predicts the impact of both large-scale structural and single base-pair variations. |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | The tool has been used in collaboration with multiple groups to understand the effect of large-scale disease-associated structural variations on genome function and gene expression. |
| URL | https://github.com/rschwess/deepC |
| Title | Hudep cell lines as a model for Congenital Dyserythropoietic Anaemia Type I (CDA-I) |
| Description | The Hudep cell lines are erythroid progenitor lines that can be induce to differentiate into erythroblasts. In separate lines we have tagged the two genes known to underlie CDA-I, CDAN1 and C15ORF41. We have also introduced one of the common CDAN1 mutations into a tagged line and now have two mutant lines for CDAN1 that faithfully replicate the diagnostic nuclear morphology of patient erythroblasts. We have now also introduced a mutation into the tagged C15ORF41 line. These cell lines means we avoid the need for mouse models. |
| Type Of Material | Model of mechanisms or symptoms - human |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | One paper on the Hudep cell lines has been published (Olijnik et al PMID 32518175). Another paper (on the ultrastructural analysis of abnormal erythroblast nuclei found in CDA-I, using the mutated Hudep cell lines) is in preparation. |
| Title | Micro Capture-C |
| Description | Base pair resolution assay for linking genes with regulatory elements |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| Impact | This tool is now recognised at the most powerful chromosome conformation capture method for linking regulatory elements with genes and decoding human genetics. It was used to decode a human haplotype associated with substantially increased mortality upon VOVID 19 infection. It has been licensed exclusively to Nucleome Therapeutics. |
| URL | https://www.nature.com/articles/s41596-023-00817-8 |
| Title | RASER-FISH |
| Description | RASER-FISH is a fluorescence in situ hybridisation method that avoids the need for denaturing DNA before a DNA probe can hybridise. In this way, chromatin structure can be optimally maintained. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | Papers published: Brackley et al 2016 PMID: 27036497 Brown et al 2018 PMID: 30242161 Oudelaar et al 2018 PMID: 30374068 Ochs et al 2019 PMID: 31645724 Rhodes et al 2020 PMID: 31968256 Miron et al 2020PMID: 32967822 Chiariello et al 2020 PMID: 32075757 A detailed methods paper has now been accepted by Nature Protocols: Brown JM, Parisi E, De Ornellas S, Schermelleh L, Buckle VJ. (2022) RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure. Nat Protocols in press. |
| Title | RASER-FISH |
| Description | RASER-FISH stands for resolution after single-strand exonuclease resection and maximally retains nuclear structure while performing FISH experiments. Published in Nature Communications/ |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | We show that this region forms an erythroid-specific, decompacted, self-interacting domain, delimited by frequently apposed CTCF/cohesin binding sites early in terminal erythroid differentiation, and does not require transcriptional elongation for maintenance of the domain structure. Formation of this domain does not rely on interactions between the a-globin genes and their major enhancers, suggesting a transcription-independent mechanism for establishment of the domain. However, absence of the major enhancers does alter internal domain interactions. Formation of a loop domain therefore appears to be a mechanistic process that occurs irrespective of the specific interactions within. |
| Title | Sasquatch |
| Description | An online version of our computational tools for high throughput prediction of the effect of SNPs on transcription factor binding in non-coding regulatory regions using DNase footprint meta-analysis |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This tool allows for the prioritization of genomic sequence variants based of the impact on the formation of DNA foorprints |
| URL | http://apps.molbiol.ox.ac.uk/sasquatch/cgi-bin/foot.cgi |
| Title | TRI-C |
| Description | TRI-C is a multiplex multiways 3C assay, published in Nature Genetics that maps the coincident and simultaneous interaction between related regulatory elements in the mammalian genome. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | TRI-C showed for the first time that regulatory elements cluster in 3D space, which has profound implications for our understanding of mammalian gene regulation. In follow up work it was used to produce a revised model for promoter competition, published in Nature Comummincations. |
| Title | Tiled-C |
| Description | Tiled-C is an adaptation of the Capture-C technologies that generate ultrdeep Hi-C like data and is applicable to very small cells numbers. At present under consideration in nature communications. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Tiled-C has revised our current understanding of the link between the regulatory structure or the genome, epigenetic activity and gene expression. |
| Title | Use of oligonucleotide probe pools to elucidate chromatin organisation |
| Description | Massive scale synthesis and novel synthetic labelling of oligonucleotide pools covering up to 3Mb of the human and mouse genomes, for use in both chromosome conformation capture and super-resolution imaging. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | Papers on: Dynamics of the 4D genome during lineage specification, differentiation and maturation in vivo (doi.org/10.1101/763763) Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin (doi.org/10.1101/566638) Targeted high-resolution chromosome conformation capture at genome-wide scale (doi.org/10.1101/2020.03.02.953745) A tissue-specific self-interacting chromatin domain forms independently of enhancer-promoter interactions (doi.org/10.1101/234427) |
| Title | scaRNA-seq |
| Description | A method to detect and quantify the amount of promoter-proximal pausing found at the level of individual genes. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | Gene transcription occurs via a cycle of linked events, including initiation, promoter-proximal pausing, and elongation of RNA polymerase II (Pol II). A key question is how transcriptional enhancers influence these events to control gene expression. Here, we present an approach that evaluates the level and change in promoter-proximal transcription (initiation and pausing) in the context of differential gene expression, genome-wide. This combinatorial approach shows that in primary cells, control of gene expression during differentiation is achieved predominantly via changes in transcription initiation rather than via release of Pol II pausing. Using genetically engineered mouse models, deleted for functionally validated enhancers of the a- and ß-globin loci, we confirm that these elements regulate Pol II recruitment and/or initiation to modulate gene expression. Together, our data show that gene expression during differentiation is regulated predominantly at the level of initiation and that enhancers are key effectors of this process. |
| URL | https://www.sciencedirect.com/science/article/pii/S1097276521000022?via%3Dihub |
| Title | DeepC: predicting 3D genome folding using megabase-scale transfer learning |
| Description | Predicting the impact of noncoding genetic variation requires interpreting it in the context of three-dimensional genome architecture. We have developed deepC, a transfer-learning-based deep neural network that accurately predicts genome folding from megabase-scale DNA sequence. DeepC predicts domain boundaries at high resolution, learns the sequence determinants of genome folding and predicts the impact of both large-scale structural and single base-pair variations. |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | The tool has been used in collaboration with multiple groups to understand the effect of large-scale disease-associated structural variations on genome function and gene expression. |
| URL | https://github.com/rschwess/deepC |
| Description | Cohesin and polycomb-dependent chromatin interactions |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We used single cell RASER-FISH analysis to investigate the effects of cohesin and PCR1 removal on chromatin interactions at specific loci. |
| Collaborator Contribution | Our collaborators Dr James Rhodes and Prof Rob Klose (Biochemistry) established the degron ES cell lines for removal of SCC1 and RING1B, identified enhanced polycomb interactions, investigated the effect on gene expression. |
| Impact | Rhodes JDP, Feldmann A, Hernández-Rodríguez B, Díaz N, Brown JM, Fursova NA, Blackledge NP, Prathapan P, Dobrinic P, Huseyin M, Szczurek A, Kruse K, Nasmyth KA, Buckle VJ, Vaquerizas JM, Klose RJ. (2020) Cohesin disrupts polycomb-dependent chromosome interactions. Cell Reports 30 (3): 820-835. |
| Start Year | 2018 |
| Description | Dark Matter Project |
| Organisation | New York University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | I have been made a member of the Dark Matter project based on our work to predict 3D genome structure using deep neural network approaches and build genome regulatory domains from scratch to understand the principles of gene regulation in the mammalian genome. |
| Collaborator Contribution | The Dark Matter Project are expert in the use of large-scale synthetic biology approaches and have agreed to build from scratch 10 complete regulatory domains guided by our machine learning approaches. These will be integrated into the mouse genome to test basic principles of mammalian gene regulation |
| Impact | The partnership has only just been initiated. |
| Start Year | 2021 |
| Description | MRC Human Genetic Unit Edinburgh |
| Organisation | Medical Research Council (MRC) |
| Department | MRC Human Genetics Unit |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | This is a scientific collaboration with Prof Nick Gilbert and Davide Marenduzzo, based around an application for a Wellcome Trust Investigator award to work on the molecular basis of 3D genome interaction that direct gene expression. |
| Collaborator Contribution | All three group are expert in different aspect of the 3D genome and have formed a collaboration to use their expertise in collaboration and to ask for funding from the Wellcome trust to support this work. |
| Impact | The collaboration has just been initiated. |
| Start Year | 2021 |
| Title | METHOD OF ANALYSING DNA SEQUENCES |
| Description | The present invention relates to a method of identifying nucleic acid regions within a nucleic acid sample which interact with one another. In particular, the method relates to a chromatin conformation capture (3C) method which may be used to analyse the interactions between enhancers, silencers, boundary elements and promoters at individual loci at high resolution. |
| IP Reference | WO2017068379 |
| Protection | Patent application published |
| Year Protection Granted | 2017 |
| Licensed | No |
| Impact | None so far |
| Title | DeepC: predicting 3D genome folding using megabase-scale transfer learning. |
| Description | Predicting the impact of noncoding genetic variation requires interpreting it in the context of three-dimensional genome architecture. We have developed deepC, a transfer-learning-based deep neural network that accurately predicts genome folding from megabase-scale DNA sequence. DeepC predicts domain boundaries at high resolution, learns the sequence determinants of genome folding and predicts the impact of both large-scale structural and single base-pair variations. |
| Type Of Technology | Webtool/Application |
| Year Produced | 2020 |
| Open Source License? | Yes |
| Impact | DeepC allows for the prediction of the effect of both large-scale and single base-pair changes of the regulatory structure of the genome and so provide sa platform for the identifications of pathogenic mutations in genome sequence. |
| URL | https://www.nature.com/articles/s41592-020-0960-3 |
| Company Name | Nucleome Therapeutics |
| Description | Nucleome Therapeutics utilises 3D genome structures with the aim of delivering genetics-based treatments. |
| Year Established | 2019 |
| Impact | Nucleome Therapeutics was spun out from the University of Oxford in 2019 after a decade of foundational research, with seed investment of £5.2m from Oxford Sciences Innovation. |
| Website | http://www.nucleome.com |
| Description | Advising Powys Dance on developing an educational ballet on The Cell |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Powys Dance ( mainly funded by the Arts Council for Wales) are developing an educational ballet to tour schools and science festivals. Last year their ballet was physics-based. This year they are focussing on gene regulation and cellular development and differentiation, all based on blood cells. They held a workshop over three days with dancers, set designers, musicians and scientific advisors where I spoke about my research and helped plan how that could be turned into dance! |
| Year(s) Of Engagement Activity | 2018,2019 |
| URL | http://dawnspowysdance.org/powys-dance-news/2018/12/21/professors-on-tour-the-lowdown |
| Description | Attendence of 42st JP Morgan Healthcare conference |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Scientific and business interaction, via Nucleome Therapeutics, with senior members of the top 10 Pharma industries. |
| Year(s) Of Engagement Activity | 2024 |
| URL | https://www.jpmorgan.com/about-us/events-conferences/health-care-conference |
| Description | Impelo performances of The Cell |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | After advising on choreography for the educational ballet Cell by Impelo (https://www.impelo.org.uk/cell) I attended several performances of the ballet in London and Oxford to take Q&A sessions after the performance. In addition, Impelo have produced supporting material in the form of a comic and explanatory videos featuring an avatar of Prof Buckle (https://www.imm.ox.ac.uk/about/news/mrc-wimm-research-shines-at-this-years2019-oxford-science-and-ideas-festival). |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.imm.ox.ac.uk/about/news/arts-meets-science-in-dance-exploration-of-cell-biology |
| Description | Invited Speaker - 14th International Conference on Genomes, Pathways, and Systems Medicine |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited Speaker - 14th International Conference on Genomes, Pathways, and Systems Medicine |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.aegeanconferences.org/src/App/conferences/view/158 |
| Description | Invited Speaker - BCGB meeting Birmingham |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited Speaker - BCGB meeting Birmingham. Presentation of research to the faculty |
| Year(s) Of Engagement Activity | 2023 |
| Description | Invited Speaker Erasmuc MC Rotterdam |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | An invited speaker to the Erasmus MC school of Hematology |
| Year(s) Of Engagement Activity | 2024 |
| Description | Invited speaker to CRUK Institute Manchester |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | A scientific presentation of research to the CRUK Institute Manchester |
| Year(s) Of Engagement Activity | 2024 |
| Description | Novartis freenovation grant board |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | I took part in the expert panel for the Novartis freenovation initiative. |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://novartisgrants.smapply.io/ |
| Description | Presentation at the Creative Machine AI symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | This was a presentation and participation in an open symposium for use of Artificial intelligence approaches in society. |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.creativemachine.io/ |
| Description | Presentation at the Dark Genome Symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at the joint industry/academia scientific meeting |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.eventcreate.com/e/dark-genome-symposium-2023/?aid=10123283 |
| Description | Royal Society Summer Science Exhibition |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | The Royal Society Summer Exhibition is a prestigious event, showcasing science from only 22 groups, so we were very pleased to be selected. It has an extremely wide audience and consequent impact, including a media day where we recorded several interviews. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.medsci.ox.ac.uk/folding-genome |
| Description | ed 41st JP Morgan Healthcare conference |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | ed 41st JP Morgan Healthcare conference. Interaction with leading Pharma industry partners |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.jpmorgan.com/solutions/cib/insights/health-care-conference |