A lifetime of attrition: post-genomic analyses of ecological and evolutionary processes in nematode - vertebrate systems.
Lead Research Organisation:
University of Liverpool
Department Name: Sch of Biological Sciences
Abstract
Virtually every species of terrestrial, freshwater and marine vertebrate is parasitized by nematodes (roundworms). Nematode infections exhibit persistent infection and re-infection throughout the life of a host, with strong sex, age and season effects on intensities of infection. These parasites therefore constitute an important component of the environment of vertebrates and affect the fitness of individuals and the dynamics of populations. There is now a timely opportunity to apply new methods from genomics to explore the ecological and evolutionary consequences of vertebrate-nematode interactions in the natural environment. Such work holds great promise but extending genomics from the bench-top to natural populations also presents significant challenges. In particular, the variation between individuals that makes research in natural populations so important also makes it difficult to apply some genomics methods, such as high throughput gene expression (microarray) analyses. Our response to these challenges is to build a consortium based around three well-characterised vertebrate - nematode systems, the Soay sheep and Teladorsagia circumcincta, the red grouse and Trichostrongylus tenuis and the brown rat and Strongyloides ratti. We will use a series of approaches ranging from microarray studies within laboratory experiments that mimic the wild situation, through to microarray studies on individuals living in wild populations subject to experimental manipulations. As a result, we hope to learn from each other, and demonstrate to the research community, just how far these new methodologies can be applied to natural populations in situ. In the process, we expect to generate a trained group of workers with the skills to develop the field more widely in the UK. Work within the consortium will explore a set of related questions that, in combination, allow us to address two broad objectives that are crucial to advancing our understanding of host-parasite ecology and evolution: (1) To dissect and quantify the link between the genetic basis of host-parasite interactions and the population biology of hosts and parasites. Infection is a process that acts on individuals but impacts on the dynamics of populations; through the effects of infection on the growth, survival and reproduction of individuals. Equally, the density of a population will impact on the level of competition between individuals for food or mates and consequently on the condition of individuals and their susceptibility to infection. The key challenge now is to understand how individual processes of infection influence, and are influenced by, the population biology of hosts and parasites. (2) To examine the origins and maintenance of genetic diversity in host and parasite populations. Within a population, individual hosts exhibit genetic differences in their susceptibility to infection. Equally, populations of parasitic nematodes are genetically variable with consequences for the persistence and transmission of infection. The key challenges to be addressed now are to identify regions of vertebrate and nematode genomes where genetic diversity is maintained through the continued interaction between both antagonists, the consequences of this genetic diversity and the selective processes by which this diversity is maintained.
Publications
Beraldi D
(2008)
Phenotypic analysis of host-parasite interactions in lambs infected with Teladorsagia circumcincta.
in International journal for parasitology
Bortolotti GR
(2009)
Physiological stress mediates the honesty of social signals.
in PloS one
Craig BH
(2009)
Re-establishment of nematode infra-community and host survivorship in wild Soay sheep following anthelmintic treatment.
in Veterinary parasitology
Craig BH
(2010)
Sex ratio and morphological polymorphism in an isolated, endemic Teladorsagia circumcincta population.
in Journal of helminthology
Evans H
(2008)
Microarray analysis of gender- and parasite-specific gene transcription in Strongyloides ratti.
in International journal for parasitology
Gratten J
(2010)
The genetic basis of recessive self-colour pattern in a wild sheep population.
in Heredity
HERNÁNDEZ-SÁNCHEZ J
(2010)
Mapping quantitative trait loci in a wild population using linkage and linkage disequilibrium analyses
in Genetics Research
Martínez-Padilla J
(2010)
Testing the interactive effects of testosterone and parasites on carotenoid-based ornamentation in a wild bird.
in Journal of evolutionary biology
Martínez-Padilla J
(2014)
Insights into population ecology from long-term studies of red grouse Lagopus lagopus scoticus.
in The Journal of animal ecology
Mougeot F
(2009)
Honest sexual signalling mediated by parasite and testosterone effects on oxidative balance.
in Proceedings. Biological sciences
Mougeot F
(2010)
Oxidative stress and the effect of parasites on a carotenoid-based ornament.
in The Journal of experimental biology
Mougeot F
(2010)
Physiological stress links parasites to carotenoid-based colour signals.
in Journal of evolutionary biology
O'Meara H
(2010)
Response of the Strongyloides ratti transcriptome to host immunological environment.
in International journal for parasitology
Pemberton JM
(2011)
Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs.
in Molecular ecology
Piertney S
(2008)
Characterising functionally important and ecologically meaningful genetic diversity using a candidate gene approach
in Genetica
Slate J
(2009)
Gene mapping in the wild with SNPs: guidelines and future directions.
in Genetica
Vergara P
(2011)
The ornament-condition relationship varies with parasite abundance at population level in a female bird.
in Die Naturwissenschaften
Vergara P
(2012)
The condition dependence of a secondary sexual trait is stronger under high parasite infection level
in Behavioral Ecology
Vergara P
(2012)
Environmental heterogeneity influences the reliability of secondary sexual traits as condition indicators.
in Journal of evolutionary biology
Vergara P
(2012)
Environmental conditions influence red grouse ornamentation at a population level Environmental Conditions and Ornaments
in Biological Journal of the Linnean Society
Webster LM
(2011)
Transcriptomic response of red grouse to gastro-intestinal nematode parasites and testosterone: implications for population dynamics.
in Molecular ecology
Webster LM
(2011)
Identification of genes responding to nematode infection in red grouse.
in Molecular ecology resources
Wenzel M
(2012)
A transcriptomic investigation of handicap models in sexual selection
in Behavioral Ecology and Sociobiology
Wenzel M
(2014)
Identification and characterisation of 17 polymorphic candidate genes for response to parasitic nematode (Trichostrongylus tenuis) infection in red grouse (Lagopus lagopus scotica)
in Conservation Genetics Resources
Description | Fundamental biology on infectious disease. |
Exploitation Route | Fundamental biology on infectious disease. |
Sectors | Environment |