Impact of stereochemistry of antimicrobial agents on their environmental fate, biological potency and the emergence of resistance
Lead Research Organisation:
University of Bath
Department Name: Chemistry
Abstract
This project aims to understand and address the impact of stereoisomerism of antimicrobial agents in their environmental cycle on mechanisms behind the development of antimicrobial resistance. The risk of promotion of antibiotic resistant bacteria is by far the greatest human health concern with regards to medicinal products in the environment. The continuous introduction of sub-inhibitory quantities of antimicrobial agents (AAs) to the environment is believed to be directly linked with antimicrobial resistance (AMR). Unfortunately, there is little knowledge of mechanisms in the environment and influencing factors due to the multi-dimensional nature of the AMR problem. There are several research gaps that need to be addressed including research into contaminated habitats (e.g. wastewater) where AAs, co-selecting agents, bacteria carrying resistance determinants and favourable conditions for bacterial growth prevail at the same time. Furthermore, the stereochemistry of AAs (which is key in defining their biological potency) has never been studied in the context of their environmental fate and effects. This is an oversight as changes in stereoisomeric profile of AAs throughout their environmental cycle will lead to (and be influenced by) changes in the composition and structure of microbial communities present in the environment. This might further contribute to the development of AMR, a phenomenon that has never been the subject of investigation in the context of stereochemistry of AAs.
This project postulates that stereochemistry of AAs determines their environmental fate and biological effects. It also hypothesizes that two enantiomers of the same AA should be recognised as two different substances that can elicit different responses leading to changes in the environmental fate and effects of the drug.
The project will:
1. Verify the mechanisms of (stereoselective) transformation of chiral antimicrobial agents and their metabolites during wastewater treatment and in receiving waters
2. Identify resistant bacterial taxa responsible for (stereoselective) degradation of antimicrobial agents and to study the development of antimicrobial resistance at stereoisomeric level
3. Recommend changes to ERA via inclusion of AAs (and their stereochemistry) and ARGs as AMR indicators
The stereochemistry of AAs is complex, as many of the semi-synthetic agents are marketed as mixtures of diastereomers and a number of synthetic agents are used as racemates. In this project we will focus on ofloxacin and chloramphenicol, but we will also consider other synthetic quinolones, Beta-lactams (e.g. amoxicillin) and carbapenems (e.g. meropenem).
Considering the importance of better understanding environmental and human health impacts from chiral pollutants such as AAs and the need for the development of new solutions tackling AMR, this project has the potential to lead to groundbreaking research with long term scientific, technological and societal impact.
This project postulates that stereochemistry of AAs determines their environmental fate and biological effects. It also hypothesizes that two enantiomers of the same AA should be recognised as two different substances that can elicit different responses leading to changes in the environmental fate and effects of the drug.
The project will:
1. Verify the mechanisms of (stereoselective) transformation of chiral antimicrobial agents and their metabolites during wastewater treatment and in receiving waters
2. Identify resistant bacterial taxa responsible for (stereoselective) degradation of antimicrobial agents and to study the development of antimicrobial resistance at stereoisomeric level
3. Recommend changes to ERA via inclusion of AAs (and their stereochemistry) and ARGs as AMR indicators
The stereochemistry of AAs is complex, as many of the semi-synthetic agents are marketed as mixtures of diastereomers and a number of synthetic agents are used as racemates. In this project we will focus on ofloxacin and chloramphenicol, but we will also consider other synthetic quinolones, Beta-lactams (e.g. amoxicillin) and carbapenems (e.g. meropenem).
Considering the importance of better understanding environmental and human health impacts from chiral pollutants such as AAs and the need for the development of new solutions tackling AMR, this project has the potential to lead to groundbreaking research with long term scientific, technological and societal impact.
Planned Impact
This project will provide cutting-edge advances in the understanding of the transformation mechanisms of antimicrobial agents and their biological effects including AMR. It will identify future research objectives directed at informed decision making on the wastewater treatment technologies used, the procedures applied in risk assessment of antimicrobial agents, the use of antimicrobial agents, relevant policies/regulations and consumer behaviours.
This project partners with a major UK water utility - Wessex Water, which will provide the opportunity for the project outcomes to be translated into effective applications within the water industry such as revision and implementation of existing and novel strategies aiming at reduction of impacts from antibiotics and AMR. In the light of the research, there may be grounds for legislative, procedural or policy changes in the area of wastewater treatment which are likely to be of significant interest to regulators. Collaboration with Environment Agency will allow for the development of a strategy aiming at limiting impacts from antibiotics and AMR.
Impact activities will include:
1. Development of inter-disciplinary and cross-sectoral research capability in AMR at the University of Bath.
2. Enhancing public-private sector collaborations: this project builds upon existing collaborations between public organisations and the private sector and will enhance these collaborations through the active involvement of the private sector in research activities. A workshop will be organised to facilitate knowledge exchange in particular in areas of pharmaceutical research, environmental management and water engineering.
3. Strengthening interdisciplinary and cross-sectoral collaborations in research: Due to its interdisciplinary nature, this project requires cross-sectoral collaborations in research both in formulating project objectives and in implementing research outcomes with an aim of measurable short and long-term impact in several AMR related areas including: water engineering, regulatory, pharmaceutical and health related sectors. This will be achieved via inclusion of key stakeholders as Project Partners representing water (Wessex Water) and regulatory (Environment Agency) sectors.
4. Communication of research outputs via publications in high IF journals, presentations at scientific conferences, popular media, articles in newspapers, webpages, presentations to the general public (e.g. Bath Science Café or Pint of Science talks), workshops and collaborative visits (to facilitate knowledge exchange between academic and public/private sector) and other outreach activities (e.g. promotion of research at schools).
5. Acquisition of key skills by the project researchers through a unique training programme that involves engagement with integrated disciplines conducting cutting edge research and exposure to private sector.
This project partners with a major UK water utility - Wessex Water, which will provide the opportunity for the project outcomes to be translated into effective applications within the water industry such as revision and implementation of existing and novel strategies aiming at reduction of impacts from antibiotics and AMR. In the light of the research, there may be grounds for legislative, procedural or policy changes in the area of wastewater treatment which are likely to be of significant interest to regulators. Collaboration with Environment Agency will allow for the development of a strategy aiming at limiting impacts from antibiotics and AMR.
Impact activities will include:
1. Development of inter-disciplinary and cross-sectoral research capability in AMR at the University of Bath.
2. Enhancing public-private sector collaborations: this project builds upon existing collaborations between public organisations and the private sector and will enhance these collaborations through the active involvement of the private sector in research activities. A workshop will be organised to facilitate knowledge exchange in particular in areas of pharmaceutical research, environmental management and water engineering.
3. Strengthening interdisciplinary and cross-sectoral collaborations in research: Due to its interdisciplinary nature, this project requires cross-sectoral collaborations in research both in formulating project objectives and in implementing research outcomes with an aim of measurable short and long-term impact in several AMR related areas including: water engineering, regulatory, pharmaceutical and health related sectors. This will be achieved via inclusion of key stakeholders as Project Partners representing water (Wessex Water) and regulatory (Environment Agency) sectors.
4. Communication of research outputs via publications in high IF journals, presentations at scientific conferences, popular media, articles in newspapers, webpages, presentations to the general public (e.g. Bath Science Café or Pint of Science talks), workshops and collaborative visits (to facilitate knowledge exchange between academic and public/private sector) and other outreach activities (e.g. promotion of research at schools).
5. Acquisition of key skills by the project researchers through a unique training programme that involves engagement with integrated disciplines conducting cutting edge research and exposure to private sector.
Publications
Castrignanò E
(2020)
(Fluoro)quinolones and quinolone resistance genes in the aquatic environment: A river catchment perspective.
in Water research
Castrignanò E
(2020)
Enantiomeric profiling of quinolones and quinolones resistance gene qnrS in European wastewaters.
in Water research
Elder FCT
(2021)
Stereoselective Bacterial Metabolism of Antibiotics in Environmental Bacteria - A Novel Biochemical Workflow.
in Frontiers in microbiology
Hernández F
(2019)
The role of analytical chemistry in exposure science: Focus on the aquatic environment.
in Chemosphere
Hernández F
(2018)
Mass spectrometric strategies for the investigation of biomarkers of illicit drug use in wastewater.
in Mass spectrometry reviews
Styszko K
(2021)
Occurrence of pharmaceutical residues, personal care products, lifestyle chemicals, illicit drugs and metabolites in wastewater and receiving surface waters of Krakow agglomeration in South Poland.
in The Science of the total environment
Description | Output 1: A new analytical method has been developed to undertake the multiresidue enantiomeric profiling of (fluoro)quinolones and their metabolites in solid and liquid environmental matrices using chiral HPLC-MS/MS method and a CHIRALCEL ® OZ-RH column. Simultaneous chiral separation was obtained for chiral ofloxacin and its main metabolites ofloxacin-N-oxide and desmethyl-ofloxacin; moxifloxacin; the prodrug prulifloxacin and its active compound ulifloxacin; flumequine; nadifloxacin and R-(þ)-besifloxacin. Achiral antibiotics (ciprofloxacin, norfloxacin and nalidixic acid) were also included in the method to enable the analysis of all targeted quinolones within one analytical run. Satisfactory enantiomeric resolution was obtained for five out of eight chiral drugs enabling quantitative analysis. The overall performance of the method was satisfactory with a method precision <20%, relative recoveries >70% for most of the analytes and method detection limits (MDL) at low ng/L levels. Enantiomeric profiling from a week-long monitoring campaign in the UK showed that (±)-ofloxacin was found to be racemic in upstream waters but it was enriched with S-(-)-enantiomer in wastewater and in receiving waters. This could be due to the fact that ofloxacin can be used both as a racemate and as a S-(-)-enantiomer. Its consumption was further confirmed by the chiral signature of the investigated ofloxacin metabolites. As a result, alterations in the enantiomeric composition of antibiotics could influence not only their activity and toxicity in the environment, but also could induce changes in the microbial communities constantly exposed to them. Output 2: This study provided an insight into the prevalence of (fluoro)quinolones (FQs) and their specific quinolone qnrS resistance gene in the Avon river catchment area receiving treated wastewater from 5 wastewater treatment plants (WWTPs), serving 1.5 million people and accounting for 75% of inhabitants living in the catchment area in the South West of England. Ofloxacin, ciprofloxacin, nalidixic acid and norfloxacin were found to be ubiquitous with daily loads reaching a few hundred g/day in wastewater influent and tens of g/day in receiving waters. This was in contrast to other FQs analysed: flumequine, nadifloxacin, lomefloxacin, ulifloxacin, prulifloxacin, besifloxacin and moxifloxacin, which were hardly quantified. Enantiomeric profiling revealed that ofloxacin was enriched with the S-(-)-enantiomer, likely deriving from its prescription as the more potent enantiomerically pure levofloxacin, alongside racemic ofloxacin. While ofloxacin's enantiomeric fraction (EF) remained constant, high stereoselectivity was observed in the case of its metabolite ofloxacin-N-oxide. The removal efficiency of quinolones during wastewater treatment at 5 WWTPs utilising either trickling filters (TF) or activated sludge (AS), was compound and wastewater treatment process dependent, with AS providing better efficiency than TF. The qnrS resistance gene was ubiquitous in wastewater. Its removal was WWTP treatment process dependent with TF performing best and resulting in significant removal of the gene (from 28 to 75%). AS underperformed with only 9% removal in the case of activated sludge and actual increase in the gene copy number within sequencing batch reactors (SBRs). Interestingly, the data suggests that higher removal of antibiotics could be linked with high prevalence of the gene (SBR and WWTP E) and vice versa, low removal of antibiotic is correlated with lower prevalence of the gene in wastewater effluent (TF, WWTP B and D). This is especially prominent in the case of ofloxacin and could indicate that AS might be facilitating antimicrobial resistance (AMR) prevalence to higher extent than TF. Wastewater based epidemiology (WBE) was also applied to monitor any potential misuse (e.g. direct disposal) of FQs in the catchment. In most cases higher use of antibiotics with respect to official statistics (i.e. ciprofloxacin, ofloxacin) was observed, which suggests that FQs management practice require further attention. |
Exploitation Route | Output 1: Analytical methods can be utilised by researchers and water/analytical/environment professionals, including regulators, to study fate and effects of chiral antibiotics. Output 2: Knowledge of enantiomer-depended processes occurring in the environment will be of importance to those working in the area of environmental and public health risks, including regulators. |
Sectors | Environment Healthcare Pharmaceuticals and Medical Biotechnology |
Description | This award has contributed to the following: • Improved Wessex Water's ability to detect and analyse a wider range of compounds and their metabolites using new rapid analytical techniques; improved sampling methods and influenced sampling sites; • Informed Wessex Water priorities including investment decisions and future areas of work i.e., the AMR programme (the choice of site for UKWIR AMR and methods); • Impact of the AMR programme to understand current sources, transformation through the sewerage system and potential risks to both wider public health and the environment. • Contributed to Wessex Water shift in focus from characterising wastewater to looking at the wider impact on the environment, ecosystem and public health • Raised awareness and understanding of the wider impact of contaminants in our sewage and receiving watercourses |
First Year Of Impact | 2019 |
Sector | Environment |
Impact Types | Policy & public services |
Description | Member of the Substances of Emerging Concern Advisory Group (SECAG), UKWIR (UK Water Industry Research) |
Geographic Reach | National |
Policy Influence Type | Participation in a guidance/advisory committee |
Description | Chemical indicators in wastewater: a model to estimate population in WBE using multi-chemical biomarker suite |
Amount | £9,934,501 (GBP) |
Organisation | UK Health Security Agency |
Sector | Public |
Country | United Kingdom |
Start | 08/2021 |
End | 02/2022 |
Description | DTG Directed, Stereochemistry of antimicrobial agents in urban water cycle and its impacts upon the evolution of antimicrobial resistance |
Amount | £89,114 (GBP) |
Funding ID | NE/R008094/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2022 |
Description | EPSRC, Project title: The fate of antimicrobials in urban wastewater and its role in the development of antimicrobial resistance |
Amount | £23,500 (GBP) |
Funding ID | 1789298 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2016 |
End | 12/2019 |
Description | Exploring understudied aspects of antimicrobial resistance evolution to improve environmental pollution policy |
Amount | £649,825 (GBP) |
Funding ID | NE/W006251/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 08/2022 |
End | 08/2025 |
Description | PAthways of Chemicals Into Freshwaters and their ecological ImpaCts (PACIFIC) |
Amount | £488,646 (GBP) |
Funding ID | NE/X015890/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 11/2022 |
End | 10/2026 |
Description | Quantifying the combined nutrient enrichment, pathogenic, and ecotoxicological impacts of livestock farming on UK rivers |
Amount | £1,182,976 (GBP) |
Funding ID | NE/X016080/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 11/2022 |
End | 10/2026 |
Description | The National CIP 2020-2021 Chem 3, Antimicrobial Resistance (AMR) |
Amount | £283,092 (GBP) |
Organisation | UK Water Industry Research Ltd |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 08/2020 |
End | 04/2022 |
Description | The Somer Valley Innovative Pathway Control (IPC) project |
Amount | £445,000 (GBP) |
Organisation | Wessex Water |
Sector | Private |
Country | United Kingdom |
Start | 11/2020 |
End | 11/2023 |
Title | New analytical methodology for enantioselective separation of quinolones in environmental matrices |
Description | New analytical methodology utilising chiral chromatography and mass spectrometry to separate chiral and non-chiral (fluoro)quinolones in environmental matrices |
Type Of Material | Improvements to research infrastructure |
Year Produced | 2018 |
Provided To Others? | Yes |
Impact | This method allowed for understanding of enantioselective processes of (fluoro)quinolones in the environment |
URL | https://www.sciencedirect.com/science/article/pii/S0045653518308476?via%3Dihub |
Title | SAM (stereoselective antimicrobial metabolism workflow |
Description | SAM (stereoselective antimicrobial metabolism) workflow is a novel interdisciplinary approach for assessing bacterial resistance mechanisms in the context of antibiotic-bacteria interactions that utilise a combination of whole genome sequencing and mass spectrometry |
Type Of Material | Technology assay or reagent |
Year Produced | 2021 |
Provided To Others? | Yes |
Impact | n/a |
URL | https://www.frontiersin.org/articles/10.3389/fmicb.2021.562157/full |
Title | Concentrations of antibiotic resistance genes in wastewater and receiving waters following water treatment in SW England, June-October, 2015 |
Description | Prevalence of quinolone qnrS resistance gene in the aquatic environment from the Avon river catchment area receiving treated wastewater from 5 wastewater treatment plants (WWTPs), serving 1.5 million people and accounting for 75% of inhabitants living in the catchment area in the South West of England. |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | Yes |
Impact | n/a |
URL | https://catalogue.ceh.ac.uk/documents/102f8141-2a9a-4ffd-89f6-961af36ddcb3 |
Title | Concentrations of antibiotics in wastewater and receiving waters following water treatment in SW England, June-October, 2015 |
Description | his dataset comprises of weekly concentrations of fluoroquinolones in wastewater and receiving waters collected in the Avon river catchment South West UK. The following fluoroquinolones were analysed: (±)-ofloxacin, (±)-ofloxacin-N-oxide, (±)-desmethyl-ofloxacin, (±)-lomefloxacin, (±)-moxifloxacin, S,S-moxifloxacin-N-sulfate, R-(+)-besifloxacin, (±)-prulifloxacin, (±)-ulifloxacin, (±)-flumequine and (±)-nadifloxacin; ciprofloxacin, desethylene-ciprofloxacin, norfloxacin and nalidixic acid. The concentrations of (fluoro)quinolones were measured in receiving treated wastewater from 5 wastewater treatment plants (WWTPs), which serves 1.5 million people accounting for 75% of inhabitants living in the catchment area in the South West of England. |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | Yes |
Impact | n/a |
URL | https://catalogue.ceh.ac.uk/documents/f4c79bd3-3ce9-4f4e-ae19-bb4fd2dce7a5 |
Description | Collaboration with Environment Agency |
Organisation | Environment Agency |
Country | United Kingdom |
Sector | Public |
PI Contribution | Collaboration within NE/N019261/1 project entitled: 'Impact of stereochemistry of antimicrobial agents on their environmental fate, biological potency and the emergence of resistance' |
Collaborator Contribution | Collaboration within NE/N019261/1 project entitled: 'Impact of stereochemistry of antimicrobial agents on their environmental fate, biological potency and the emergence of resistance' |
Impact | Ongoing |
Start Year | 2016 |
Description | Collaboration with Wessex Water |
Organisation | Wessex Water |
Country | United Kingdom |
Sector | Private |
PI Contribution | expertise, equipment, facilities |
Collaborator Contribution | expertise, equipment, facilities |
Impact | Several, including academic papers: Petrie, B., Proctor, K., Youdan, J., Barden, R. and Kasprzyk-hordern, B., 2017. Critical evaluation of monitoring strategy for the multi-residue determination of 90 chiral and achiral micropollutants in effluent wastewater. Science of the Total Environment, 579, pp. 569-578. Petrie, B., Gravell, A., Mills, G. A., Youdan, J., Barden, R. and Kasprzyk-Hordern, B., 2016. In situ calibration of a new chemcatcher configuration for the determination of polar organic micropollutants in wastewater effluent. Environmental Science & Technology, 50 (17), pp. 9469-9478. Petrie, B., Youdan, J., Barden, R. and Kasprzyk-Hordern, B., 2016. A new framework to diagnose the direct disposal of prescribed drugs in wastewater - a case study of the antidepressant fluoxetine. Environmental Science & Technology, 50 (7), pp. 3781-3789. Petrie, B., Youdan, J., Barden, R. and Kasprzyk-Hordern, B., 2016. Multi-residue analysis of 90 emerging contaminants in liquid and solid environmental matrices by ultra-high-performance liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 1431, pp. 64-78. Petrie, B., Barden, R. and Kasprzyk-Hordern, B., 2015. A review on emerging contaminants in wastewaters and the environment:current knowledge, understudied areas and recommendations for future monitoring. Water Research, 72, pp. 3-27. |
Start Year | 2012 |
Description | 3. Cartwright, N and Kasprzyk-Hordern, B. To understand the risks to the environment from chemicals, , Environment Agency meeting 'Working Together for a Better Environment', Bristol, 21st February 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Industry/Business |
Results and Impact | Talk on collaboration between University of Bath, Wessex Water and Environment Agency |
Year(s) Of Engagement Activity | 2017 |
URL | https://www.instituteofwater.org.uk/events-info/613/ |
Description | Barbara Kasprzyk-Hordern, WBE and One Health, HRMS EnviroFood 2022 Barcelona, HRMS EnviroFood 2022 Barcelona , 10/10/22 ? 11/10/22, Barcelona, Spain (invited talk) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Barbara Kasprzyk-Hordern, WBE and One Health, HRMS EnviroFood 2022 Barcelona, HRMS EnviroFood 2022 Barcelona , 10/10/22 ? 11/10/22, Barcelona, Spain (invited talk) |
Year(s) Of Engagement Activity | 2022 |
URL | https://lcmsms.activacongresos.com/ |
Description | Barbara Kasprzyk-Hordern, Wastewater surveillance of community-wide antimicrobials usage, International association of Environmental and Analytical Chemistry - Waste Water Analysis: Environmental and Epidemiological Implications, International Association of Environmental and Analytical Chemistry, 2022, 1/06/22 (invited talk) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Barbara Kasprzyk-Hordern, Wastewater surveillance of community-wide antimicrobials usage, International association of Environmental and Analytical Chemistry - Waste Water Analysis: Environmental and Epidemiological Implications, International Association of Environmental and Analytical Chemistry, 2022, 1/06/22 (invited talk) |
Year(s) Of Engagement Activity | 2022 |
Description | Innovation through partnership working, DEFRA, London 15th November 2016. |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Talk at DEFRA WQ Team away day |
Year(s) Of Engagement Activity | 2016 |
Description | Kasprzyk-Hordern, Antibiotics and resistance genes in the environment, University of Nottingham Sutton Bonington AMR Webinar Series 2020 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Talk by Kasprzyk-Hordern, Antibiotics and resistance genes in the environment, University of Nottingham Sutton Bonington AMR Webinar Series 2020 |
Year(s) Of Engagement Activity | 2020 |
Description | Kasprzyk-Hordern, interview, BBC Radio Wales: Science Cafe, 8th of September 2020 |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Radio interview |
Year(s) Of Engagement Activity | 2020 |
URL | https://tinyurl.com/y52g4bld |
Description | Public talk entitled: 'Healthy waters: Pharmaceuticals in the environment - cause for concern? Bath Festival of Nature, 2nd June 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Public talk delivered by Kasprzyk-Hordern. Title: Healthy waters: Pharmaceuticals in the environment - cause for concern? Bath Festival of Nature, 2nd June 2017. |
Year(s) Of Engagement Activity | 2017 |
Description | Talk by B. Kasprzyk-Hordern, 'Urban water fingerprinting for environmental and public health assessmen', Kwaluzu-Natal University, Durban, 1st of February 2019, South Africa |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Talk by B. Kasprzyk-Hordern, 'Urban water fingerprinting for environmental and public health assessmen', Kwaluzu-Natal University, Durban, 1st of February 2019, South Africa (invited talk) |
Year(s) Of Engagement Activity | 2019 |
Description | Talk by B. Kasprzyk-Hordern, My career in water, 18th UK Young Water, Professionals Conference, University of Bath, 10 -12th April 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Talk by B. Kasprzyk-Hordern, My career in water, 18th UK Young Water, Professionals Conference, University of Bath, 10 -12th April 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Talk by Kasprzyk-Hordern 'Antimicrobials in water cycle: Research gaps', Israel-UK Synergy programme grant symposium and workshop, 25th and 26th April 2018:"One Health: antimicrobial resistance from mechanisms to interdisciplinary solutions" The Institute of Microbiology and Infection, University of Birmingham |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Kasprzyk-Hordern, B., Castrignano E, Edler, F, 'Antimicrobials in water cycle: Research gaps', Israel-UK Synergy programme grant symposium and workshop, 25th and 26th April 2018:"One Health: antimicrobial resistance from mechanisms to interdisciplinary solutions" The Institute of Microbiology and Infection, University of Birmingham (invited talk) |
Year(s) Of Engagement Activity | 2018 |
Description | Talk by Kasprzyk-Hordern, 'Urban water fingerprinting to inform the state of the environment and public health', University College London Chemical & Physical Society Guest Lecturer |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Undergraduate students |
Results and Impact | B. Kasprzyk-Hordern, 'Urban water fingerprinting to inform the state of the environment and public health', University College London Chemical & Physical Society Guest Lecturer 16th October 2018 (invited talk) |
Year(s) Of Engagement Activity | 2018 |
Description | Talk by Kasprzyk-Hordern, B., What's Your water telling you? Urban water profiling inform the state of the environment and public health, RSC Event, Bristol, 13th Feb 2018, University of the West of England |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Kasprzyk-Hordern, B., What's Your water telling you? Urban water profiling inform the state of the environment and public health, RSC Event, Bristol, 13th Feb 2018, University of the West of England (invited talk) |
Year(s) Of Engagement Activity | 2018 |
Description | Talk by Kasprzyk-Hordern, Pharmaceuticals in the environment - cause for concern?, Viewpoints Keynsham: Healthy Water, 23rd of March 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Public talk by Kasprzyk-Hordern, Pharmaceuticals in the environment - cause for concern?, Viewpoints Keynsham: Healthy Water, 23rd of March 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Talk delivered by Kasprzyk-Hordern, entitled: Water fingerprinting for public health assessment', Wetenschappelijke Advies Raad / Scientific Advisory Council KWR, Nieuwegein, the Netherlands, 1st of June 2017 |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Industry/Business |
Results and Impact | Talk delivered by Kasprzyk-Hordern, entitled: Water fingerprinting for public health assessment' at Wetenschappelijke Advies Raad / Scientific Advisory Council KWR, Nieuwegein, the Netherlands, 1st of June 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | WBE for drug use and public health assessment' Amsterdam International Water Week Conference, 30 October - 3 November 2017, Amsterdam |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Talk delivered by Kasprzyk-Hordern entitled: 'WBE for drug use and public health assessment' at Amsterdam International Water Week Conference |
Year(s) Of Engagement Activity | 2017 |
Description | Water fingerprinting for environmental and public health assessment, Centre of Research in Biomedical Science (CRIB), UWE, 4th December 2020 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Talk by Kasprzyk-Hordern focussed on 'Water fingerprinting for environmental and public health assessment', Centre of Research in Biomedical Science (CRIB), UWE, 4th December 2020 |
Year(s) Of Engagement Activity | 2020 |
Description | What's Your water telling you? Urban water profiling inform the state of the environment and public health |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Public talk delivered by Kasprzyk-Hordern B entitled: 'What's Your water telling you? Urban water profiling inform the state of the environment and public health', RSC Event, Bristol, 13th Feb 2018, University of the West of England |
Year(s) Of Engagement Activity | 2017 |