Lab-on-a-Paper for Point-of-Use Microbial Source Tracking
Lead Research Organisation:
University of Glasgow
Department Name: School of Engineering
Abstract
Water contamination with microbial organisms in water systems, including drinking, ground, recreational, and wildlife, is a global issue. Even with well-operated drinking-water treatment plants, such as those available in Scotland and the UK, drinking water distribution systems are vulnerable to episodic pathogen intrusion (from pressure losses, repairs or rain-induced run-off of dirty water), presenting high risks to human health and significant economic losses. For example the Centre for Disease Control in the United States (one of the safest drinking water systems in the world) estimate that there are 4-32 million cases of acute gastrointestinal illness per year from public drinking water systems. Contamination is also impacting local distribution systems with decentralised facilities, such as those present in many low and middle-income countries (LMICs) as well as remote areas of 'developed' countries (such as Scotland). In this Fellowship, low-cost, deployable paper-based biosensor devices (lab-on-a-paper) will be developed for the online monitoring of water quality.
Using sensors built around simple and low-cost paper-based devices to process water samples, we will develop rapid, sensitive and easy-to-use testing devices that can detect genetic markers of pathogens, along with co-markers of their origins, a key capability to identify multiple pathogens in drinking water and track their source. For example, the detection of human DNA markers could potentially infer a human faecal contamination, while animal genes (e.g. cattle, pig, etc...) could help identify agricultural sources. By deploying these portable sensors, we will obtain data on contamination patterns and dynamics, which in turn will provide the ability to not only rapidly respond to the contamination and curtail it before consumption of drinking water, but also design new surveillance systems and build new understandings of the pathways taken by the contaminating pathogens in the environment.
The detection of microbial contamination together with its tracing and tracking in the environment is currently performed mainly by isolating, culturing and identifying the pathogens against known contaminants, through a long process that can take many days and extensive technical expertise. New procedures have been improved to enable faster testing via the molecular detection of specific genetic markers or the pathogens (< 1 hour), but these currently require centralised facilities and skilled personnel, preventing their use in the field. The technologies developed in this Fellowship will allow the detection of multiple genetic markers rapidly, in the field. These highly multiplexed assays will provide the capability for industry to provide a rapid and dynamic response to a contamination, and to identify and track its source.
Working with Scottish Water, we will validate the devices in the field in Scotland. Our close collaboration will ensure that the developments are relevant to the end-users, such that the translation into practical use can be accomplished with minimal delay and risk. This will have the potential to enable "sustainable communities and sustainable homes", an initiative of Scottish Water for small rural communities which is particularly important in Scotland as well as affording opportunities for growth internationally.
Beyond rural communities in high-resources settings, decentralised water systems are also present in low and middle-income countries. Through interactions with NGOs, we will aim to explore the impact of the technologies developed in this Fellowship in the wider community, globally.
In future, our biotechnological platform will also enable source tracking and monitoring in the wider environment (e.g. agricultural processes), including antimicrobial resistance, thus providing a cornerstone in solving challenges arising to maintain a healthy population, a key strand of the UK Industrial Strategy.
Using sensors built around simple and low-cost paper-based devices to process water samples, we will develop rapid, sensitive and easy-to-use testing devices that can detect genetic markers of pathogens, along with co-markers of their origins, a key capability to identify multiple pathogens in drinking water and track their source. For example, the detection of human DNA markers could potentially infer a human faecal contamination, while animal genes (e.g. cattle, pig, etc...) could help identify agricultural sources. By deploying these portable sensors, we will obtain data on contamination patterns and dynamics, which in turn will provide the ability to not only rapidly respond to the contamination and curtail it before consumption of drinking water, but also design new surveillance systems and build new understandings of the pathways taken by the contaminating pathogens in the environment.
The detection of microbial contamination together with its tracing and tracking in the environment is currently performed mainly by isolating, culturing and identifying the pathogens against known contaminants, through a long process that can take many days and extensive technical expertise. New procedures have been improved to enable faster testing via the molecular detection of specific genetic markers or the pathogens (< 1 hour), but these currently require centralised facilities and skilled personnel, preventing their use in the field. The technologies developed in this Fellowship will allow the detection of multiple genetic markers rapidly, in the field. These highly multiplexed assays will provide the capability for industry to provide a rapid and dynamic response to a contamination, and to identify and track its source.
Working with Scottish Water, we will validate the devices in the field in Scotland. Our close collaboration will ensure that the developments are relevant to the end-users, such that the translation into practical use can be accomplished with minimal delay and risk. This will have the potential to enable "sustainable communities and sustainable homes", an initiative of Scottish Water for small rural communities which is particularly important in Scotland as well as affording opportunities for growth internationally.
Beyond rural communities in high-resources settings, decentralised water systems are also present in low and middle-income countries. Through interactions with NGOs, we will aim to explore the impact of the technologies developed in this Fellowship in the wider community, globally.
In future, our biotechnological platform will also enable source tracking and monitoring in the wider environment (e.g. agricultural processes), including antimicrobial resistance, thus providing a cornerstone in solving challenges arising to maintain a healthy population, a key strand of the UK Industrial Strategy.
Publications
Mao K
(2021)
Biosensors for wastewater-based epidemiology for monitoring public health.
in Water research
Mao K
(2020)
An integrated biosensor system with mobile health and wastewater-based epidemiology (iBMW) for COVID-19 pandemic.
in Biosensors & bioelectronics
Ming T
(2020)
Paper-based microfluidic aptasensors.
in Biosensors & bioelectronics
Nnachi RC
(2022)
Biosensors for rapid detection of bacterial pathogens in water, food and environment.
in Environment international
Ou Y
(2021)
Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology.
in Trends in analytical chemistry : TRAC
Pan Y
(2022)
Paper-based devices for rapid diagnosis and wastewater surveillance
in TrAC Trends in Analytical Chemistry
Pena-Pereira F
(2021)
Miniaturized analytical methods for determination of environmental contaminants of emerging concern - A review.
in Analytica chimica acta
Teng D
(2020)
Efficient removal of Cd(II) from aqueous solution by pinecone biochar: Sorption performance and governing mechanisms.
in Environmental pollution (Barking, Essex : 1987)
Wang Y
(2020)
Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR.
in Microsystems & nanoengineering
Wang Y
(2022)
Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases.
in Medicine in novel technology and devices
Description | Through the past year of research, we have developed a new paper-based device for organism detection in drinking water. This platform allows for the multiplexed detection of several microbial contaminations within less than 1 hour. The sensing device is very cheap and easy-to-use. Apart from this, we have also developed a paper-microfluidic electrochemical biosensor for the rapid detection of protein biomarker, however, this electrochemical aptamer sensor can be adapted to detect a range of chemical contaminants for rapid monitoring of water quality. Furthermore, we developed a sensing platform for the detection of illicit drugs and virus in wastewater to support the national wastewater surveillance monitoring programme |
Exploitation Route | This sensors platform has significant advantages of fast response time (from sample to answer less than 1 hour), cost-effective and user-friendly. We have developed paper-based sensors, allowing for chemical and microbial contaminants detection in water. This could be of significant help for the industry in the water sector, for example, our industry partner Scottish Water. |
Sectors | Agriculture Food and Drink Chemicals Environment Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
Description | We started to engage with the water utility company to explore wide application with our developed device for rapid and cost-effective monitoring. |
First Year Of Impact | 2019 |
Sector | Chemicals,Environment,Healthcare |
Description | Lab-on-a-Paper for Point-of-Use Microbial Source Tracking |
Amount | £266,568 (GBP) |
Funding ID | NE/R013349/2 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 03/2019 |
End | 07/2021 |
Description | National COVID-19 Wastewater Epidemiology Surveillance Programme |
Amount | £791,191 (GBP) |
Funding ID | NE/V010441/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 06/2020 |
End | 11/2021 |
Description | Paper-based platform for on site, rapid, and multiplexed DNA-based pathogen detection in aquaculture |
Amount | £161,462 (GBP) |
Funding ID | BB/S004335/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 12/2018 |
End | 03/2020 |
Title | Raman sensors- WR-2021 |
Description | Experimental data for the article |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | Yes |
Impact | Provide temporal and spatial data sets for illicit drug use as a literature reference. |
URL | https://figshare.com/articles/dataset/_/13173251 |
Title | Apparatus and Method for Rapid Monitoring of Pathogens in Water |
Description | It provides a rapid and on-site method for the enrichment and detection of pathogens in water and wastewater. |
IP Reference | |
Protection | Patent application published |
Year Protection Granted | 2022 |
Licensed | No |
Impact | It provides a rapid and on-site method for the enrichment and detection of pathogens in water and wastewater. |
Description | Researchers Seek a Simple, Rapid Test for SARS-CoV-2 in Sewage |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Media (as a channel to the public) |
Results and Impact | Onsite testing at wastewater treatment plants could aid efforts to monitor for outbreaks of COVID-19 around the world, but such technologies are in the early stages of development. |
Year(s) Of Engagement Activity | 2020 |