Device for Enrichment of Skeletal Stem Cells for Orthopaedic Applications
Lead Research Organisation:
University of Southampton
Department Name: Development Origin of Health and Disease
Abstract
With an increasing ageing population, strategies that allow the simple repair and augmentation of bone tissue, lost as a consequence of trauma or disease or with ageing, are urgently needed.The ability to develop a device that would allow the simple enrichment of skeletal stem cells from the bone marrow of a patient for orthopaedic application is thus an unmet and urgently required clinical target. This project proposes to progress the development of a unique and patented cell separation technology, developed by Smith and Nephew into a clinically effective and commercially viable surgical device. The implications for orthopaedic use are immense and potential for improving the quality of life in an increasing ageing population significant.This novel technology has the potential to transform orthopaedic clinical practice by increasing the accessibility of enriched autologous marrow. The Southampton group, includes clinicians and basic scientists, that will evaluate the device using human bone marrow samples and, through experimental studies aid in the evaluation and, if necessary, additional development of the cell separation device. The ability of the device to enrich for skeletal stem cells will be assessed by a battery of assays as well as unique models of bone formation available within Southampton.The collaboration between academic, industrial and clinical researchers will allow rapid development of the cell enrichment device and, critically, evaluation with human tissue to include pre-clinical study. This will allow us, within a 2 year time frame, to significantly contribute in Southampton to the programme of work from S&N to the generation of the regulatory data needed to allow clinical use of the proposed device.
Publications
Tayton ER
(2012)
Translational hurdles for tissue engineering: an in vitro analysis of commonly used local anaesthetics on skeletal stem cell survival.
in The Journal of bone and joint surgery. British volume
Tayton ER
(2013)
Effects of setting bone cement on tissue-engineered bone graft: a potential barrier to clinical translation?
in The Journal of bone and joint surgery. American volume
Tayton E
(2014)
A comparison of polymer and polymer-hydroxyapatite composite tissue engineered scaffolds for use in bone regeneration. An in vitro and in vivo study.
in Journal of biomedical materials research. Part A
Tayton E
(2012)
An analysis of polymer type and chain length for use as a biological composite graft extender in impaction bone grafting: a mechanical and biocompatibility study.
in Journal of biomedical materials research. Part A
Smith JO
(2014)
Tantalum trabecular metal - addition of human skeletal cells to enhance bone implant interface strength and clinical application.
in Journal of tissue engineering and regenerative medicine
Smith JO
(2011)
Skeletal tissue regeneration: current approaches, challenges, and novel reconstructive strategies for an aging population.
in Tissue engineering. Part B, Reviews
Dawson JI
(2014)
Concise review: bridging the gap: bone regeneration using skeletal stem cell-based strategies - where are we now?
in Stem cells (Dayton, Ohio)
Dawson JI
(2013)
Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration.
in Cytotherapy
Title | "An understanding of the apparatus of bodily production in the stem cell and regenerative medicine space |
Description | A series of artworks, photographs and a book on "An understanding of the apparatus of bodily production in the stem cell and regenerative medicine space Developed over 2010 to 2012 and curated in 2013 |
Type Of Art | Artwork |
Year Produced | 2013 |
Impact | "An understanding of the apparatus of bodily production in the stem cell and regenerative medicine space" Formed a major exhibition- a series of artworks and a book |
URL | https://www.southampton.ac.uk/medicine/business_partnership/success_stories/intra_actions_and_reconf... |
Description | We have developed a strategy to enrich for bone stem cells with the development of a cell sorter. We have also built on previous work, in this study, that a bone graft with bone cells would result in a living bone composite and could find clinical application. |
Exploitation Route | Our bone stem cell enrichment device could be developed and used in theatres throughout the NHS and wider. Our continued observation of the ability of bone cells combined with bone graft to create a living bone composite that aids bone regeneration could be used in a range of situations where additional bone stock is required. |
Sectors | Healthcare |
Description | We have developed new approaches to create a living bone graft composite for clinical application. Specifically work, in part, from this grant as well as previous work, allowed the development of the concept of a bone graft combined with bone cells resulted in a bioactive construct that could find clinical application. |
First Year Of Impact | 2008 |
Sector | Healthcare |
Impact Types | Societal |
Description | National Science Week |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | Yes |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Several thousand visitied University of Southampton Science event- the Bone and Joint Musculoskeletal Activity drew approx 200-300 individuals who activelty participated (made models, discussed bone repair, handeled samples) in the last science week exhibition. Candidates asked specifically about Medicine Bachelor of Medicine programmes and how to apply. Patients asked if they could jopin clinical trials in the future as availalble for impaction bone grafting - so extremely positive feedback for the researchers on this project. |
Year(s) Of Engagement Activity | 2009,2010,2011,2012,2013,2015,2016 |
Description | Scaffolds and cells - making replacement body parts in the lab - Exhibit at the BBSRC Great British Bioscience Festival |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Engaged over 6500 visitors from across the UK as part of the BBSRC run GBBF. EPSRC grant generated the centre piece of our exhibit - the Stem cell mountain - a large interactive pinball machine built in collaboration with Winchester Science Centre to communicate the concept of Stem Cell Potential. In response to the feedback question 'Tell us something from your visit that you found particularly interesting' the answer 'Stem Cells' was one of the most frequently given. Through a conversation at this exhibition a talented international MSc student from Queen Mary's was inspired to commence a PhD project (self-funded) in my group. |
Year(s) Of Engagement Activity | 2014 |