Long-range control of tissue-specific expression in Arabidopsis

Lead Research Organisation: University of Warwick
Department Name: School of Life Sciences


Gene expression regulation is critical for all living organisms. Functional analyses of genomic architecture have revealed the existence of regulatory elements residing distal to coding sequences. To understand how the combined activity of these regulatory elements affects the temporal and special regulation of gene activity, it is essential to define the constitutive elements of gene regulation. Understanding how this long-distance regulation occurs and uncovering the mechanisms involved in this process are two fundamental unresolved questions in biology. Contrary to the situation in animals, we remain ignorant of the role played by long-distance regulatory sequences in gene expression regulation in plants. The main objective of this study is to identify and characterise the sequences that are involved in the spatial and temporal regulation of gene expression in the experimental model plant, Arabidopsis thaliana, and how they are influenced by environmental fluctuations. We will elucidate specific gene function through the targeted manipulation of regulatory elements and genome-wide analyses. This knowledge will be critical to engineer crop species that are better adapted to environmental stress.


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Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M01116X/1 30/09/2015 29/09/2023
1643084 Studentship BB/M01116X/1 04/10/2015 29/09/2019 Nosheen Hussain
Description I mangled to to devise a new method called in vivo NOME-seq to study open chromatin in plants. The technique is powerful enough to identify DNAS regulatory landscape and identified regions can be further modified genetically or epigenetically to study distal regulation.
Exploitation Route The technique can be effectively used in different crops t o study chromatin and be subsequently used to enhance our understanding of gene regulation. With the help of this method we can identify important features in the crop genome that can be modified to enhance crop yield and over come negative impact of diseases and environmental stresses.
Sectors Agriculture, Food and Drink

Description 3 Day Master class "Genome Editing using CRISPR/Cas9" 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Master class was intended to deliver a hands on experience of Genome editing in the lab as well as in the form of lecture to year 1 PhD students across the students from 3 universities i.e. UniversityWarwick, University of Birmingham and Leicester University. The master class was part of the Midlands Integrative Bioscience programme training for year1 PhD students. Participants were able to learn how to delete target gene using CRISPR/Ca9 using Arabidopsis protoplast cells starting from basic cloning and finally genotyping the edited genome.
Year(s) Of Engagement Activity 2018