Decoding the endothelial adhesome - a sticky business

Lead Research Organisation: University of East Anglia
Department Name: Graduate Office

Abstract

Angiogenesis is the process by which new blood vessels form from pre-existing ones. It occurs during embryonic development and is essential for maintaining healthy physiological processes such as wound healing and the restructuring of the uterine wall during the menstrual cycle. This process works less effectively as we age and mis-regulation of angiogenesis contributes to a multitude of malignant, inflammatory, ischaemic, infectious and immune disorder. Thus increasing understanding of angiogenesis regulation and allowing manipulation of it to maintain health and repair disease throughout life.

The lab is interested in the role endothelial B3-integrin plays in the angiogenic process; endothelial cells are the key cellular mediators of angiogenesis. Using quantitative mass spectrometry the initial stages of this project will extend the laboratory's current analysis of the endothelial adhesome by examining changes that occur within this complex molecular network when B3-integrin expression is depleted. We will then focus on B3-integrin-dependent molecular pathways to determine their functional role in mediating endothelial migration in response to angiogenic stimuli.

During this project, the student will develop expertise in a wide range of cross-disciplinary techniques, including working with knockout mice, endothelial cell isolation, cell imaging, tissue culture, immunohistochemistry, and biochemical assays including mass spectrometry and Western blot analyses whilst keeping abreast of rapidly changing literature so that findings from other studies and our own lab can be communicated effectively and in a timely manner.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M011216/1 01/10/2015 30/09/2023
1654326 Studentship BB/M011216/1 01/10/2015 30/09/2019 Robert Johnson
 
Description Deciphering the angiogenic tug-of-war between neuropilin-1 interacting integrins: phase II
Amount £182,222 (GBP)
Funding ID PG/17/46/32880 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2018 
End 02/2020
 
Description Student Member Bursary
Amount £160 (GBP)
Organisation Biochemical Society 
Sector Learned Society
Country United Kingdom
Start 03/2018 
End 04/2018
 
Description Angiogenesis GRS 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Oral Presentation on research funded by this award at the 2017 Angiogenesis Gordon Research Seminar, Rhode Island, USA
Year(s) Of Engagement Activity 2017
 
Description Imperial Vascular Endothelium Symposium 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Oral Presentation on research funded by this project to participants of the 2017 Imperial Vascular Endothelial Symposium, London, UK
Year(s) Of Engagement Activity 2017