Investigating the roles of CDA and DCTD in cancer nucleotide metabolism
Lead Research Organisation:
University of Oxford
Department Name: Clinical Medicine
Abstract
I am investigating THE role salvage enzymes play in the nucleotide acquisition pathway in cancer cells.
I am doing this through use of 2D cell culture, protein level manipulation and novel mass spectrometric techniques I have developed.
To date, I have set up two mass spectrometric methods, together with the Kessler group at the TDI. The first is capable of sensitive DNA and RNA global composition analysis. The second directly measures the nucleotide pool composition.
I will use parental cells that have stable enzyme knock-in and knockdown daughter cells and monitor the effect siRNA knockdowns of CDA and DCTD have on cellular metabolism.
I will perform this on a panel of cells to avoid cell bias.
I am doing this through use of 2D cell culture, protein level manipulation and novel mass spectrometric techniques I have developed.
To date, I have set up two mass spectrometric methods, together with the Kessler group at the TDI. The first is capable of sensitive DNA and RNA global composition analysis. The second directly measures the nucleotide pool composition.
I will use parental cells that have stable enzyme knock-in and knockdown daughter cells and monitor the effect siRNA knockdowns of CDA and DCTD have on cellular metabolism.
I will perform this on a panel of cells to avoid cell bias.
Organisations
People |
ORCID iD |
| Paolo Spingardi (Student) |
Publications
Motnenko A
(2018)
Identification of UHRF2 as a novel DNA interstrand crosslink sensor protein.
in PLoS genetics
Müller CA
(2019)
Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads.
in Nature methods
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| MR/N013468/1 | 30/09/2016 | 29/09/2025 | |||
| 1790304 | Studentship | MR/N013468/1 | 30/09/2016 | 31/12/2020 | Paolo Spingardi |
| Title | Mass Spectrometry |
| Description | Agilent 6495 QQQ for nucleoside and nucleotide quantification. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | 3 collaborations that have led to paper submissions, more in the pipeline. |