Programable DNA roadblocks using CRISPR-Cas complexes
Lead Research Organisation:
University of Nottingham
Department Name: School of Life Sciences
Abstract
To utilize CRISPR-Cas "interference" complexes, Cascade, Cas9 and Cpf1 as programmable blocks to DNA replication in vitro and in vivo. In the rotation, the complexes will be expressed to guide RNA to specificed targets in DNA and we will begin to determine if the complexes are effective at blocking DNA replication and/or gene transcription.
Organisations
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M008770/1 | 01/10/2015 | 31/10/2024 | |||
| 1796150 | Studentship | BB/M008770/1 | 01/10/2016 | 31/03/2021 |
| Description | STEM Open Day (Nottingham) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Myself and a group of other PhD students attended an open day hosted by a series of schools in Nottingham to be attended by the general public (largely drawn from Nottinghamshire). There we presented posters which gave a basic overview of our work and were available by the posters to discuss our work with school-children, parents, the general public and some undergraduate students who also attended. My own experience was that I was able to clear up some general misconceptions about gene-editing held by many members of the public and to generate interest in post-graduate study for undergraduate students that I spoke to. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Visit (Derby) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Myself and two other PhD students made a visit to a school where we presented some background to our own work and its importance in an accessible manner. This presentation was made to approx. 40 pupils from a high-achieving Biology class at a school in Derby. The same pupils then undertook a short workshop in-class which was aimed at showing them the diversity of microbes that can be found from different sources in nature such as water from a nearby lake, soil and the surface of fruits and cheeses. This involved the pupils being able to plate out some of these things on agar plates which were then stored until a later visit where they were inspected for growth. After the session another short presentation and Q&A session was provided to answer the pupils' questions about working in science and to encourage them to work towards degrees and careers in science where possible. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Science Festival - Festival of Curiosity |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I took part in the festival of curiosity as part of a group from the Bolt lab, demonstrating through a small practical exercise the process of DNA mutation, to the public at the event. I was also able to discuss with the public about the importance of research in DNA mutation / editing and its significance in illness. The intended purpose was to raise awareness of research in this area and to demonstrate to the public that it is valuable in a variety of areas in science. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://nottsfosac.co.uk/event/curiosity-market/ |