Regulation of root attachment in Rhizobium - AfS, ENWW

Lead Research Organisation: University of Oxford
Department Name: Interdisciplinary Bioscience DTP

Abstract

The attachment to legume roots of soil rhizobia represents the first key stage of physical interaction between host plants and future endosymbionts in nitrogen fixing Rhizobiumlegume symbioses. It is these symbioses that give the plants a 'self-fertilizing' phenotype, and can largely free them from synthetic fertilizer requirements. This is important, as synthetic fertilizers are both expensive and environmentally polluting. Complex genetic switches, controlled in a precise spatio-temporal fashion, are required to enable the appropriate activation of root attachment factors in both the eukaryote and prokaryotes. The production by Rhizobium of exopolysaccharide (EPS) is particularly important for this interaction. Previous work has identified a sigma factor (EcfE) in Rhizobium leguminosarum biovar viciae 3841, which acts as a master regulator of pea root attachment, as well as a diguanylate cyclase (RL2880) important for EPS production. Building on these findings, this work aims to expand knowledge of the genetic regulation surrounding root attachment, using mutagenesis screens and other in vitro techniques to reveal new regulatory hierarchies. It is hoped that a greater understanding of this area may contribute to enhancing the efficiency of these symbioses, or even transferring them to non-legume plants, thereby contributing to sustainability in agriculture as well as global food security.

BBSRC priority areas addressed by this research -
Food, Nutrition and Health
Sustainably Enhancing Agricultural Production
Synthetic Biology
Global Food Security

Publications

10 25 50

Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M011224/1 01/10/2015 30/09/2023
1810140 Studentship BB/M011224/1 01/10/2015 30/09/2019 Jack David Parsons
 
Description Federation of European Microbiological Societies Grants
Amount € 350 (EUR)
Organisation Federation of European Microbiological Societies (FEMS) 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2017 
End 07/2017
 
Description Bring and share pulse lunch 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Bring and share pulse lunch to celebrate world pulse day 18th Jan 2017
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Development of Poole Lab website (Rhizosphere.org) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The Rhizosphere website is used to describe work performed, profiles lab members, lists outreach activities and celebrates achievements. Also includes a list of Lab publications.
Year(s) Of Engagement Activity 2014,2015,2016,2017,2018,2019,2020
URL https://rhizosphere.org
 
Description Magazine publication - The Biologist 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Publication in bi-monthly magazine 'The Biologist', an official publication of the Royal Society of Biology. This cover story on biotechnology and engineering nitrogen fixing symbioses led to the issue being dedicated to plant sciences research.
Year(s) Of Engagement Activity 2017
URL https://thebiologist.rsb.org.uk/biologist/158-biologist/features/1791-can-we-fix-it
 
Description Posters at ICNF, Granada, Spain, Sept. 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact International conference. 11 posters from Lab. 2 short presentations
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Rothamsted Research invited seminar - Phil Poole 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Phil gave a lunch-time research seminar.
Year(s) Of Engagement Activity 2019
URL https://rhizosphere.org/lab-news/
 
Description Talk at 2nd Plant Microbiome Conference, Amsterdam, Feb 2018 (Jack Parsons) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Selected to give a talk at an international conference on Plant Microbiome
Year(s) Of Engagement Activity 2018
 
Description Talk at Nitrogen Network one-day meeting in Oxford, July 2018 (Rachel Wheatley, Euan James, Jack Parsons, Kyle Grant) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Talks were presented to audience of scientists in UK studying similar organisms and related subject areas.
Year(s) Of Engagement Activity 2018
URL https://synthsym.org/nitrogen-network/
 
Description Twitter account Rhizosphere @PooleLabOxford 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Twitter account to publicise the work of the Poole Lab
Year(s) Of Engagement Activity 2017,2018,2019,2020
URL https://twitter.com