Quantitative Drug Imaging in Living Cells using Advanced Raman Imaging Microscopy.
Lead Research Organisation:
University of Strathclyde
Department Name: Pure and Applied Chemistry
Abstract
Scientists involved in pharmaceutical drug discovery need a quantitative imaging technique that will locate the drug and the target, ideally in a living cell. Currently Mass Spectrometry is widely used for drug imaging in frozen cells in an ultra-tight vacuum; however, quantification of such measurements is very difficult. We propose to quantify the amount of drug substance in a living cell using advanced Raman scattering techniques such as stimulated Raman scattering (SRS) that will use near infra-red lasers to image the cells with <1um spatial resolution without hampering the regular cellular functions, and spectroscopic Raman methods to confirm the measurements. The methods will facilitate early detection of failure of a drug, saving significant cost and accelerate assessment of efficacy of drug uptake.
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
EP/P51066X/1 | 01/10/2016 | 30/09/2021 | |||
1811690 | Studentship | EP/P51066X/1 | 01/10/2016 | 31/03/2021 | Caitlin Thomson |