Photoactivated metal nanoparticles for gene-targeted DNA damage

Lead Research Organisation: University of Birmingham
Department Name: School of Chemistry

Abstract

In this project we will use gold nanoparticles coated with selected DNA sequences and iridium probes to test whether they are able to be specifically toxic to the DNA of cells expressing target DNA sequences. Specific uptake will be assessed using microscopy techniques and phototoxicity assessed using biochemical assays for viability, cellular ROS and DNA damage. We will design photoactivated iridium complexes to be attached to gold nanoparticles and use DNA sequences to attempt to specifically target the mutated cells. Localisation of the nanoparticles will be studied with microscopies and the effect of two-photon photoactivation of the iridium probe will be studied using cell based toxicity assays for DNA damage and oxidative stress.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M01116X/1 01/10/2015 30/09/2023
1900512 Studentship BB/M01116X/1 02/10/2017 30/09/2021 Sarah Akponasa
 
Description Three different luminescent iridium complexes made. One is novel. They show potential as imaging agents for microscopy.
Exploitation Route The complexes can be used to image cells, especially when put onto scaffolds such as gold nanoparticles that promote localisation in targeted cells.
Sectors Chemicals,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology