Activation of environmental carcinogens in human tissue organoid cultures
Lead Research Organisation:
King's College London
Department Name: Analytical & Environmental Sciences
Abstract
Hypothesis 1: Human tissue organoid cultures are good model systems to study the metabolic activation of environmental carcinogens and their genotoxicity
Hypothesis 2: Events that follow DNA damage are also key to identifying organ specificity of environmental carcinogens. Organoid cultures allow us to investigate carcinogen organotropism.
Hypothesis 2: Events that follow DNA damage are also key to identifying organ specificity of environmental carcinogens. Organoid cultures allow us to investigate carcinogen organotropism.
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| MR/N013700/1 | 01/10/2016 | 30/09/2025 | |||
| 1930789 | Studentship | MR/N013700/1 | 01/10/2017 | 31/12/2021 | Angela Caipa Garcia |
| Description | Adduct analysis |
| Organisation | University of Minnesota |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | I provided DNA from treated organoids. |
| Collaborator Contribution | They provided the analysis of DNA adducts for my project. These were also included in publications. |
| Impact | 10.3390/ijms24010606 |
| Start Year | 2020 |
| Description | Aflatoxin DNA adducts |
| Organisation | Johns Hopkins University |
| Department | Johns Hopkins Bloomberg School of Public Health |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | I provided DNA from treated organoids |
| Collaborator Contribution | DNA adduct analysis was provided. |
| Impact | Adduct data was included in my thesis and a manuscript in being prepared. |
| Start Year | 2020 |
| Description | High Throughput qRT-PCR |
| Organisation | Karlsruhe Institute of Technology |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | N/A |
| Collaborator Contribution | Analysis of organoid samples by High-throughput qRT-PCR |
| Impact | Results are being analysed, data not published yet |
| Start Year | 2019 |
| Description | Kidney organoids |
| Organisation | Princess Máxima Center |
| Country | Netherlands |
| Sector | Hospitals |
| PI Contribution | N/A |
| Collaborator Contribution | They provided kidney organoids for my project |
| Impact | Experiments still on-going |
| Start Year | 2019 |