Development of a Microfluidic Probe for High-Content Single Cell Analysis

Lead Research Organisation: University of Glasgow
Department Name: School of Engineering

Abstract

The golden standard in assessment of cellular function and state is by biochemical analysis such as qPCR, ELISA etc. Usually these measurements are based on whole cell populations of 1000s to 1000000s of cells. As there is a natural variance between cells, it is therefore of interest to analyse samples from smaller populations to single cells. As the analysis generally is acquired from a Petri dish or similar it is difficult to reduce the number of cells without reducing the concentration of the analysis too. However, with the use of a microfluidic probe, it is possible to sample directly in a Petri dish from a select population. Using this method it will be possible to sample from micro-array samples for higher throughput.

In this project we will develop a microfluidic probe platform based on an inverted microscope. This will involve the design of new probes and manipulation mechanics. Once assembled, it will be applied on a range of cell system to validate the process.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/N509668/1 01/10/2016 30/09/2021
1944397 Studentship EP/N509668/1 01/10/2017 01/10/2021 Neil Convery
 
Description 3D printed inlays can be used to as inlays in an industrial injection moulding process to provide a high-throughput fabrication of microfluidic devices. Furthermore, the moulded devices are capable of maintaining liver organoids for organ-on-a-chip applications.
Exploitation Route Fabrication method developed already put to use by others in the lab and devices are being made and sent to other collaborators.
Sectors Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology
URL https://www.sciencedirect.com/science/article/pii/S2590007219300036