Increasing lifespan in carrots: determining the molecular and metabolic mechanisms that underpin the blackening phenomenon in cut carrots
Lead Research Organisation:
University of Leeds
Abstract
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People |
ORCID iD |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/R505535/1 | 30/09/2017 | 29/09/2021 | |||
| 1947433 | Studentship | BB/R505535/1 | 30/09/2017 | 29/09/2021 |
| Description | James Hutton Institute, Dundee Scotland |
| Organisation | James Hutton Institute |
| Department | Cell and Molecular Sciences |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | I collaborated with the James Hutton Institute by discussing my project in detail and analysed my samples using their equipment and expertise. |
| Collaborator Contribution | Members of the James Hutton Institute gave me guidance and access to their facilities and equipment in order to carry out a metabolomics analysis of my samples using both HPLC-MS and GCMS. I also was shown how to use software called Excalibur to analyse my data and was given guidance on how to correctly statistically assess my results. |
| Impact | Metabolite profiling analysis was performed on blackened and control carrot samples, using a combination of Gas Chromatography and Mass Spectrometry. Significantly decreased levels of amino acids and primary sugars and carbohydrates were found in blackened samples compared to control samples. Significantly increased levels of fatty acids and non-primary sugars/carbohydrates compounds were found in blackened samples compared to control samples. Significantly increased levels of several phenolic compounds were found in blackened samples compared to control samples, however most were not significantly different. This collaboration is within one discipline. |
| Start Year | 2018 |
| Description | Luke Institute, Finland |
| Organisation | Natural Resources Institute Finland |
| Country | Finland |
| Sector | Public |
| PI Contribution | I discussed my project in detail and there have been talks of further experiments concerning my samples. I was able to use the institutes facilities and expertise's to analyse the lignin content of my samples. Further experiments have since been carried out on my remaining samples after I left and I may be visiting again to carry out more myself. |
| Collaborator Contribution | Members of the Luke Institute gave me guidance and access to their facilities and equipment in order to compare the lignin content of my samples, as well as analyse the subunit portions of the lignin using pyrolysis-GC. After I left, this lab also carried out a carbohydrate comparison using methanolysis-GC-FID. |
| Impact | A significant increase in lignin content was seen in blackened carrot tissue compared to that of orange carrot tissue. An analysis of the subunit portions of the lignin showed no significant differences. Whereas the preliminary results from the carbohydrate comparison show several differences, though more replicates are required for a statistical analysis. This collaboration is within one discipline. |
| Start Year | 2019 |