Lung function and damage over the life course and the effects of chemical agents
Lead Research Organisation:
University of Liverpool
Department Name: Institute of Infection and Global Health
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
Julian Hiscox (Primary Supervisor) | |
Abbie-Jasmine Harrison (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
MR/R502339/1 | 30/09/2017 | 30/08/2022 | |||
2115207 | Studentship | MR/R502339/1 | 30/09/2017 | 30/03/2021 | Abbie-Jasmine Harrison |
Description | DiMeN - Flexible funding for skill building |
Amount | £7,000 (GBP) |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 10/2018 |
Title | MinION sequencing |
Description | MinION is a handheld sequencing tool developed by the UK company Oxford Nanopore. MinION is portable, real-time, and comes with a relatively low-price-tag (the starter pack costs $1,000). The device contains an array of micro scaffolds that support a polymer membrane containing thousands of embedded nanopores. Once loaded and connected to a power source, an ionic current pass through each nanopore generating a voltage difference across the membrane. During sample preparation "adapter" proteins are ligated to the ends of genomic DNA or cDNA strands. The adaptor proteins direct the strands to the membrane where they pass through the pore in a unidirectional flow. As the nucleic acids are transported through a pore, each base disrupts the current in a unique fashion; allowing the sequence to be obtained. By 2014, the MinION, a hand-held portable sequencing device was introduced to an early access scheme, and in 2015 it became commercially available. During library preparation, genetic material within the sample is not intentionally fragmented, enabling the MinION to generate sequence reads which are substantially longer than other comparative platforms. Traditional sequencing methods rely on the fragmentation and in sillico reassembly of DNA/RNA sequences. This fingerprinting approach can sometimes yield poorly constructed, low quality assemblies, especially when it comes to assembling complex genomic regions and highly identical sequences which can lead to missing information and genomic reduction. Longer read lengths allow for overlapping so that the sequence can be unambiguously reconstructed. Long read lengths also enable the identification of epigenetic modifications and transcript isoforms. Apart from human applications, other organisms such as plants have highly repetitive sequences making it difficult to obtain a complete genome using short read methods. Aside from longer read lengths, MinION also has the advantage of being able to directly sequence native RNA and DNA enabling the detection of individual nucleotide modifications. |
Type Of Material | Technology assay or reagent |
Year Produced | 2019 |
Provided To Others? | No |
Impact | N/A |
URL | https://nanoporetech.com/ |
Description | Blue Dot science festival |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | At the start of each day, two people dressed in full hazmat regalia (White paper suit, shoe covers, blue gloves and facemasks) stood at a point on the Bluedot map and infected 250 passers-by. Each infected person received an infected blue sticker, and a sticker pack with instructions to infect 3 people then pass the stickers on. we mapped the spread of the Bluedot fever and identified the source point. This activity sparked questons and discussions about outbreak reponse |
Year(s) Of Engagement Activity | 2006,2019 |
URL | https://www.discoverthebluedot.com/ |