Development of a bedside diagnostic tool to determine low levels of free haemoglobin in whole blood

Lead Research Organisation: Imperial College London
Department Name: Dept of Chemistry

Abstract

Haemoglobin (Hb) is predominantly localised within the cellular compartment of red blood cells.
However, traumatic injuries, surgery and some disease states (e.g. sickle cell, thalassemia and malaria)
cause red cell rupture/haemolysis which releases free Hb into the circulation. Endogenous protection
is afforded by the Hb binding and removal protein haptoglobin (Ha), but this reserve is rapidly
overwhelmed. Free Hb has recently been implicated in kidney failures, infection, sepsis, acute
respiratory distress syndrome (ARDS), hypertension and pulmonary arterial hypertension. Thus there
is an emerging need to develop a sensitive and rapid, bedside, free Hb quantitation method to help
inform diagnosis and clinical management of patients. In this project, we will explore two potential
solutions to this challenge one based on electrochemical detection and the other spectrophotometry.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/S023518/1 01/10/2019 31/03/2028
2278913 Studentship EP/S023518/1 01/10/2019 30/09/2023 Owen Tudor Griffith