Investigating the transcriptional and functional stress responses in different neuron subtypes after knockdown of mitochondrial complex I subunit ND-7
Lead Research Organisation:
King's College London
Department Name: Clinical Neuroscience
Abstract
We hypothesise that mitochondrial complex I inhibition (via knock down of subunit ND-75) will lead to distinct transcriptional and functional stress responses in different neuronal subtypes. To test this hypothesis in the next upcoming months I plan to:
1. Analyze the behavioural effects of ND-75 KD in a single neuronal subtype, dopaminergic and serotonergic neurons.
Climbing and seizure assays
CAFÉ feeding assay
ANY-Maze open field assay
Hot plate assay
Analysis of ATF4 expression- adult brain dissections, staining and confocal microscopy
2. Analyze the transcriptional response caused by ND-75 KD in single neurons.
-We will perform single nuclear RNA-seq (snRNA-seq) from flies with pan-neuronal ND-75 KD and ND-75 KD together with ATF4 KD.
3. Test whether targeting mitochondrial stress signalling modifies dopaminergic and serotonergic neuron function.
1. Analyze the behavioural effects of ND-75 KD in a single neuronal subtype, dopaminergic and serotonergic neurons.
Climbing and seizure assays
CAFÉ feeding assay
ANY-Maze open field assay
Hot plate assay
Analysis of ATF4 expression- adult brain dissections, staining and confocal microscopy
2. Analyze the transcriptional response caused by ND-75 KD in single neurons.
-We will perform single nuclear RNA-seq (snRNA-seq) from flies with pan-neuronal ND-75 KD and ND-75 KD together with ATF4 KD.
3. Test whether targeting mitochondrial stress signalling modifies dopaminergic and serotonergic neuron function.
Organisations
People |
ORCID iD |
| Joseph Bateman (Primary Supervisor) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| NC/V001884/1 | 01/10/2021 | 30/09/2024 | |||
| 2779104 | Studentship | NC/V001884/1 | 01/10/2021 | 30/09/2024 |