High resolution cryo-electron microscopy of clathrin cage complexes

Lead Research Organisation: University of Warwick
Department Name: School of Life Sciences

Abstract

Publications

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Morris KL (2019) Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly. in Nature structural & molecular biology

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Smith S (2017) Weak Molecular Interactions in Clathrin-Mediated Endocytosis in Frontiers in Molecular Biosciences

 
Description As a result of this collaborative visit we have developed new methods to discover the structure of cage complexes using 3D electron microscopy
Exploitation Route We are using these methods currently to obtain high resolution structures of cage complexes. Once we have completed development these methods may be of use to others working with related samples.
Sectors Pharmaceuticals and Medical Biotechnology

 
Description Alert14
Amount £1,500,000 (GBP)
Funding ID BB/M01228X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2015 
End 12/2015
 
Description Clathrin assembly regulation of glucose metabolism
Amount £491,534 (GBP)
Funding ID BB/V001434/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2020 
End 11/2023
 
Description Project grant - Exploiting direct electron detection to resolve protein-protein interactions in clathrin-mediated endocytosis
Amount £550,000 (GBP)
Funding ID BB/N008391/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2016 
End 03/2019
 
Title Sorting of cage types for single particle analysis 
Description Using artificial cage types we have developed a methodology for sorting heterogeneous cage types within our clathrin sample to obtain 3D structures. 
Type Of Material Improvements to research infrastructure 
Year Produced 2019 
Provided To Others? Yes  
Impact Shortly after publication in Nature Structural and Molecular Biology (Morris et al 2019) a preprint came out in BiRXiv using this method and referencing this work. 
URL https://github.com/kylelmorris/Morris_2019_NSMB
 
Description Improving image analysis of clathrin cage complexes 
Organisation University of California, San Francisco
Country United States 
Sector Academic/University 
PI Contribution Biochemical knowledge of the system and sample preparation
Collaborator Contribution Use of cryo-electron microscope and direct electron detector. Advice on image processing approaches.
Impact As a result of the collaboration we were able to obtain preliminary data for a full project grant application to BBSRC which was successful. We have also now succeeded in publishing our work in Nature Structural and Molecular Biology (Morris et al, 2019)
Start Year 2013
 
Description Royal Society of Biology Blog 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I wrote a short blog for the Royal Society of Biology explaining my research to a general audience. Informal feedback from colleagues and via social media was positive.
Year(s) Of Engagement Activity 2016
URL https://blog.rsb.org.uk/clathrin-maintaining-cell-health-in-geometric-style/