High resolution cryo-electron microscopy of clathrin cage complexes
Lead Research Organisation:
University of Warwick
Department Name: School of Life Sciences
Abstract
USA
People |
ORCID iD |
Corinne Smith (Principal Investigator) |
Publications
Dannhauser PN
(2017)
CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function.
in The Journal of biological chemistry
Smith SM
(2017)
Weak Molecular Interactions in Clathrin-Mediated Endocytosis.
in Frontiers in molecular biosciences
Morris KL
(2019)
Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly.
in Nature structural & molecular biology
Description | As a result of this collaborative visit we have developed new methods to discover the structure of cage complexes using 3D electron microscopy |
Exploitation Route | We are using these methods currently to obtain high resolution structures of cage complexes. Once we have completed development these methods may be of use to others working with related samples. |
Sectors | Pharmaceuticals and Medical Biotechnology |
Description | Alert14 |
Amount | £1,500,000 (GBP) |
Funding ID | BB/M01228X/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2015 |
End | 12/2015 |
Description | Clathrin assembly regulation of glucose metabolism |
Amount | £491,534 (GBP) |
Funding ID | BB/V001434/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2021 |
End | 11/2024 |
Description | Project grant - Exploiting direct electron detection to resolve protein-protein interactions in clathrin-mediated endocytosis |
Amount | £550,000 (GBP) |
Funding ID | BB/N008391/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 03/2016 |
End | 03/2019 |
Title | Sorting of cage types for single particle analysis |
Description | Using artificial cage types we have developed a methodology for sorting heterogeneous cage types within our clathrin sample to obtain 3D structures. |
Type Of Material | Improvements to research infrastructure |
Year Produced | 2019 |
Provided To Others? | Yes |
Impact | Shortly after publication in Nature Structural and Molecular Biology (Morris et al 2019) a preprint came out in BiRXiv using this method and referencing this work. |
URL | https://github.com/kylelmorris/Morris_2019_NSMB |
Description | Improving image analysis of clathrin cage complexes |
Organisation | University of California, San Francisco |
Country | United States |
Sector | Academic/University |
PI Contribution | Biochemical knowledge of the system and sample preparation |
Collaborator Contribution | Use of cryo-electron microscope and direct electron detector. Advice on image processing approaches. |
Impact | As a result of the collaboration we were able to obtain preliminary data for a full project grant application to BBSRC which was successful. We have also now succeeded in publishing our work in Nature Structural and Molecular Biology (Morris et al, 2019) |
Start Year | 2013 |
Description | Inaugural lecture |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Other audiences |
Results and Impact | Delivered professorial inaugural lecture to at least 50-100 people in person and online that presented my research and career path to a broad audience. The lecture stimulated questions and positive feedback and will be reported in the departmental newsletter. |
Year(s) Of Engagement Activity | 2022 |
Description | Royal Society of Biology Blog |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | I wrote a short blog for the Royal Society of Biology explaining my research to a general audience. Informal feedback from colleagues and via social media was positive. |
Year(s) Of Engagement Activity | 2016 |
URL | https://blog.rsb.org.uk/clathrin-maintaining-cell-health-in-geometric-style/ |