Development and application of real time biosensors
Lead Research Organisation:
John Innes Centre
Department Name: Molecular Microbiology
Abstract
Biosensors are devices of biological origin that can detect the presence of chemicals or other physical factors (e.g. light, temperature) in the environment. We are proposing to develop a whole new group of chemical biosensors, in particluar for sugars and simple ions like zinc, manganese and phosphate. These biosensors are examples of nanotechnology because they can sense chemicals at the atomic to cellular level. The particular biosensors we are proposing to develop are based on simple proteins called solute binding proteins. These proteins can be fused to two other proteins (CFP and YFP) to generate a fluorescent signal when they bind a specific chemical, which in this case will be a sugar or an ion. These nanosensors can be incorporated into living cells where they will report in real time on the concentration of the chemical they can sense. This is very important because it means changes in concentrations (flux) of chemicals can be measured. Many signalling mechanisms in biology depend on changes in the concentrations of metabolites so being able to sense these changes is crucial to many fundamental studies on biological action.
Technical Summary
Biosensors are powerful tools for detection and monitoring of compounds in the environment and in the chemical and pharmaceutical industries. They can be found in a number of forms including induction biosensors, electrical biosensors and FRET biosensors. These nanosensors operate at the atomic to microscopic/cellular level, providing the ability to detect the presence of compounds as well as in some cases rates of flux of compounds. Building on our isolation of a whole new suite of solute binding protein (SBP) induction biosensors, we now propose to develop a targeted group of these into FRET biosensors. FRET biosensors allow the real time monitoring of compounds in living cells and permit rates of flux to be measured. In particular we propose to express selected sugar, ion and pharmaceutically important SBPs and measure their ligand binding affinity. They will then be expressed as eCFP-SBP-eYFP fusion proteins for measurement and optimisation of their FRET signals. In addition 6-10 of the most novel of the SBPs will be crystallised to enable rational modification of their ligand specificity and FRET optimisation. In a radical new development we also propose to develop a targeted metagenomic library for the isolation of novel induction biosensors. This library will be composed of genomic DNA fragments from a selection of sequenced plant and environmental bacteria. We are confident such an approach can revolutionise the whole area of isolation of novel induction biosensors. In future work this technique could be applied to almost any compound of interest and provides extraordinary selective power.
Organisations
- John Innes Centre (Lead Research Organisation)
- University of Wisconsin-Madison (Collaboration)
- James Hutton Institute (Collaboration)
- ABERYSTWYTH UNIVERSITY (Collaboration)
- University of Calcutta (Collaboration)
- Massachusetts Institute of Technology (Collaboration)
- Montana State University (Collaboration)
- University of Hyderabad (Collaboration)
- UNIVERSITY OF OXFORD (Collaboration)
- Indian Council of Agricultural Research (Collaboration)
- John Innes Centre (Collaboration)
- Plant Biology Division (Collaboration)
- THE ENERGY AND RESOURCES INSTITUTE (Collaboration)
- Oxford India Centre for Sustainable Development (OICSD) (Collaboration)
- Maharaja Sayajirao University of Baroda (Collaboration)
Publications
Tett AJ
(2012)
Regulatable vectors for environmental gene expression in Alphaproteobacteria.
in Applied and environmental microbiology
Ramachandran VK
(2011)
Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics.
in Genome biology
Poole P
(2017)
Shining a light on the dark world of plant root-microbe interactions.
in Proceedings of the National Academy of Sciences of the United States of America
Poole P
(2018)
Rhizobia: from saprophytes to endosymbionts.
in Nature reviews. Microbiology
Pini F
(2017)
Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root Secretion.
in Plant physiology
East AK
(2008)
Biosensors for ligand detection.
in Advances in applied microbiology
Description | Specialised proteins called solute binding proteins (SBPs) are responsible for uptake of specific chemical(s) by bacterial cells. SBPs have an innate ability to recognize their solute due to their intrinsic protein structure. The fact that on binding the chemical the protein changes shape means they can be used to develop very precise and specific methods of detecting that chemical. By adding fluorescent tags to a number of SBPs we developed several fluorescence resonance energy transfer (FRET) biosensors to detect sugars, dicarboxylates and aromatic compounds which can be used in a variety of situations. |
Exploitation Route | The biosensors developed could be used in research to provide real-time information on the presence and concentration of the target solute. |
Sectors | Agriculture Food and Drink Chemicals Healthcare Manufacturing including Industrial Biotechology |
Description | BioSciences DTP, University of Oxford |
Amount | £80,000 (GBP) |
Funding ID | 1211269 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2022 |
Description | Continuation of ENSA (RL) |
Amount | $226,932 (USD) |
Organisation | Foreign Commonwealth and Development Office (FCDO) |
Sector | Public |
Country | United Kingdom |
Start | 01/2021 |
End | 03/2024 |
Description | ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS |
Amount | £1,146,829 (GBP) |
Funding ID | BB/L011484/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2013 |
End | 08/2018 |
Description | India-UK Nitrogen Fixation Centre (IUNFC) |
Amount | £1,680,948 (GBP) |
Funding ID | BB/N013387/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2016 |
End | 12/2018 |
Description | Marie Curie (IA) |
Amount | € 224,933 (EUR) |
Organisation | Marie Curie |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 05/2021 |
End | 06/2023 |
Description | Phyloquant Metagenomic Identification and Quantification Technology (Pathfinder) |
Amount | £13,000 (GBP) |
Funding ID | BB/N016335/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 12/2015 |
End | 05/2016 |
Description | Spatial and temporal mapping of the pea root secretome and its control of bacterial rhizosphere colonisation |
Amount | £407,126 (GBP) |
Funding ID | BB/K001868/2 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2013 |
End | 09/2015 |
Description | Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture (Co-I with Prof. Ray Dixon as Lead PI) |
Amount | £1,624,659 (GBP) |
Funding ID | BB/N013476/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 02/2016 |
End | 01/2019 |
Description | Brazil and nitrogen fixation |
Organisation | Aberystwyth University |
Department | Institute of Biological, Environmental and Rural Sciences (IBERS) |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | Brazil and nitrogen fixation |
Organisation | James Hutton Institute |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | Brazil and nitrogen fixation |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | Gut microbiome |
Organisation | University of Oxford |
Department | Department of Psychiatry |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Analysis of the microflora of the rat gut. |
Collaborator Contribution | Study of the effects of the periphery on brain biology, memory and mood. Diet and gut bacteria increase body levels of glutamate receptor stimulating amino acids. Research is now testing if these amino acids and intestinal microbes affect brain neurotransmission and behaviour. The goal is to find the best ways to help the treatment of psychiatric disorders, and maintain healthy brain function during aging. |
Impact | Award of BBSRC grant 'The effect of early-life prebiotic feeding on adult rat hippocampal function, central and peripheral metabonomics and microbial metagenomics' Grant Ref: BB/N010035/1 |
Start Year | 2015 |
Description | India and nitrogen fixation |
Organisation | Indian Council of Agricultural Research |
Department | ICAR - Indian Agricultural Research Institute, New Delhi |
Country | India |
Sector | Private |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Indian Council of Agricultural Research |
Department | ICAR-Indian Institute of Soil Science |
Country | India |
Sector | Learned Society |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | James Hutton Institute |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Maharaja Sayajirao University of Baroda |
Department | Department of Bio-Chemistry |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Oxford India Centre for Sustainable Development (OICSD) |
Country | United Kingdom |
Sector | Public |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | The Energy and Resources Institute |
Country | India |
Sector | Charity/Non Profit |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | University of Calcutta |
Department | Department of BioChemistry |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | University of Hyderabad |
Department | Department of Plant Sciences |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | SynSym Project |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Massachusetts Institute of Technology |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Montana State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Plant Biology Division |
Country | United States |
Sector | Private |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | University of Wisconsin-Madison |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | 1st Plant Microbiome Symposium, Brazil Feb 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | 1st Plant Microbiome Symposium, Brazil attended by approx. 200 people |
Year(s) Of Engagement Activity | 2016 |
Description | 21st Congress on Nitrogen Fixation - 10th-15th Oct 2019, Wuhan, China - Philip Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a talk at this international conference. He had many questions on his work and spent time exchanging ideas with colleagues in this research area. |
Year(s) Of Engagement Activity | 2019 |
URL | http://2019icnf.csp.escience.cn/dct/page/65580 |
Description | BASF Symposium 'Unlocking Yield Potential in Soil', Germany, October 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | keynote/invited speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk provided plenty of questions from audience. Increase interest in my work. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Back2Roots Advisor, annual project meeting. Jan 2017 |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | International advisor to the Back2Roots research programme in the Netherlands |
Year(s) Of Engagement Activity | 2016 |
Description | Bacterial World, Natural History Museum, Oxford, Dec 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Phil gave a talk to school children on an open day at the museum as part of the Bacterial World exhibition. |
Year(s) Of Engagement Activity | 2018 |
URL | http://www.oum.ox.ac.uk/bacterialworld/ |
Description | Bayer Crop Science 16th-17th Feb 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Bayer Crop Science 16th-17th Feb 2016. A group of academics advised industry |
Year(s) Of Engagement Activity | 2016 |
Description | Botanic Gardens seminar |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | I talked to the Botanic Gardens about nitrogen and humanity Lots of questions and feedback. This is widely advertised to the public |
Year(s) Of Engagement Activity | 2014 |
Description | Bring and share pulse lunch |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Bring and share pulse lunch to celebrate world pulse day 18th Jan 2017 |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Centre of Excellence for Plant and Microbial Science (CEPAMS), Shanghai, China - Philip Poole (Oct 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil visited Dr Jeremy Murray at CEPAMS and gave a talk to scientists and students. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Development of Poole Lab website (Rhizosphere.org) |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | The Rhizosphere website is used to describe work performed, profiles lab members, lists outreach activities and celebrates achievements. Also includes a list of Lab publications. |
Year(s) Of Engagement Activity | 2014,2015,2016,2017,2018,2019,2020 |
URL | https://rhizosphere.org |
Description | ENFC, Budapest posters and talks |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | An international conference. Group members presented posters and gave talks to approx. 500 scientists |
Year(s) Of Engagement Activity | 2016 |
URL | https://rhizosphere.org/lab-news/ |
Description | European Nitrogen Fixation Conference (ENFC), Spain, Sept 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | keynote/invited speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk led to plenty of discussion. The six posters from the group which were also presented were well attended and provoked interest. Improved collaboration with international colleagues. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Facebook LIVE video by Oxford Sparks |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Oxford Sparks FacebookLIVE podcast on nitrogen and the importance of nitrogen-fixing legumes |
Year(s) Of Engagement Activity | 2016 |
URL | https://www.facebook.com/OxSparks/videos/1210777428999882/ |
Description | FacebookLIVE - the future of food |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Schools |
Results and Impact | Facebook LIVE podcast in association with Oxford Sparks (latest 6.6k views) |
Year(s) Of Engagement Activity | 2017 |
URL | https://www.facebook.com/OxSparks/videos/1210777428999882/ |
Description | Gatsby Plant Science Summer School, Hawkshill, July 2018 (Phil Poole, Vinoy Ramachandran) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Undergraduate students |
Results and Impact | Summer school for undergraduates interested in Plant Sciences. Phil gave a talk. Vinoy attended as a post-doc mentor to the undergraduates. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Give peas a chance |
Form Of Engagement Activity | A magazine, newsletter or online publication |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | We made a film with Oxford sparks on nitrogen fixation Lots os hits on the web site |
Year(s) Of Engagement Activity | 2014 |
Description | Graduate School Speaker, Lunteren, The Netherlands, April 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Graduate School Speaker, Lunteren, The Netherlands, April 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | KEYS conference, Amsterdam, June 2018 (Phil Poole) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | Gave a talk to an industry-sponsored conference. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Lecture at Cirencester Science and Technology Society, Royal Agricultural University, 9th May 2018, (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Lecture given to general public interested in science. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Max Planck Institute for Plant Breeding Research, Cologne, Germany Dec 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Seminar and discussion with colleagues at Max Planck Institue. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Meeting minds Oxford Alumni |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I gave a talk to the Oxford biological sciences Alumni Enthusiastic response from the audience |
Year(s) Of Engagement Activity | 2014 |
Description | Michigan State University, student workshop |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Student workshop at an American University where I was an international expert. |
Year(s) Of Engagement Activity | 2016 |
Description | Mini-symposium Chemical Signalling in the Rhizosphere (Cumbria, Sept. 2019)- Phil Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A group of international scientists focussed on a very defined problem in a retreat-like setting. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | NIAB-East Malling Research seminar series, Jan 2019 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Lunch time seminar at Research Institute. Spent much of the day in discussion with colleagues there. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Organised OxBacNet meeting - Feb 2018 (Alison East) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | University of Oxford, Bacteriology Symposium and Network meeting. Held three times a year. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/oxbacnet/ |
Description | OxBacNet presentation - Feb 2021 (LC) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Laura gave a talk entitled 'Where should I invest my resources? How host plants regulate interactions with bacterial symbionts'. This was well received (by Zoom) and she answered the audience's questions. |
Year(s) Of Engagement Activity | 2021 |
URL | https://rhizosphere.org/oxbacnet/ |
Description | Oxford Botanic Gardens Seminar Series, Nov 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Evening seminar to interested members of general public. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Plant-Microbe Interactions Symposium, Society for Applied Microbiology, Oct 2020 (PSP) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Phil gave a talk entitled 'Rhizobium from rhizosphere to root nodule' at this online conference. It reached a wide audience who asked a series of questions after the talk. |
Year(s) Of Engagement Activity | 2020 |
URL | https://sfam.org.uk/career/ems-event-calendar/plant-microbe-interactions-series-part-1.html |
Description | Research Seminar, Lisbon, March 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Research Seminar, Lisbon, March 2017 at II Semana Bioengenharia, Instuto Superior Tecnico, Lisbon, Portugal |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Rothamsted Research invited seminar - Phil Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a lunch-time research seminar. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016, addressed audience made up of academic colleagues and post-grad students |
Year(s) Of Engagement Activity | 2016 |
Description | Schools one week Intensive course |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | Intensive one week course for A level students in practical plant and microbial science Students all keen to apply for science based university courses |
Year(s) Of Engagement Activity | 2014 |
Description | Seminar, University of Southampton, Feb 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Seminar, Crop Systems Engineering Seminar Series, University of Southampton, Feb 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Talk at Curtin University, Western Australia - Phil Poole (Nov 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a seminar on his research to an audience of scientists. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Talk to Zoological Society of London, London Zoo,13th March 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Seminar to an audience interested in science, but not specialists in the field. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Thatcher Development Programme's Foundation Day Panel Discussion, 'Climate Change: Approaches and Innovations for the Future' - Feb 208 (Phil Poole) |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Undergraduate students |
Results and Impact | Broad-ranging panel discussion with students from different disciplines held at Somerville College, Oxford. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | The Microbiome: human medicine and agriculture in a bacterial world, Royal Society, Oct 2018 (Phil Poole) |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Royal Society organised two days of discussion to formulate policy. |
Year(s) Of Engagement Activity | 2018 |
Description | Twitter account Rhizosphere @PooleLabOxford |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Twitter account to publicise the work of the Poole Lab |
Year(s) Of Engagement Activity | 2017,2018,2019,2020 |
URL | https://twitter.com |
Description | University of York, Dept. of Biology invited seminar- Feb 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Seminar and active discussion with members of Dept. of Biology |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017 |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Visit to Centre of Rhizobium Studies, Murdoch University, Perth - Philip Poole (Nov 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Phil visited CRS and gave a seminar. Also during his visit he was able to interact with post-graduate students. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Visit to Murdoch University, WA |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Industry/Business |
Results and Impact | Spent 1 month in Australia as Sir Walter Murdoch Adjunct Professor with Australian colleagues. Liaising, gave a seminar and got involved with their research activities. |
Year(s) Of Engagement Activity | 2016 |
Description | Winchester College, Nov 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Phil gave a talk to the students of Winchester College. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Wow! How? Family Science Fair, March 2013, 2014, 2015 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | Yes |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Demonstrations stimulated discussion about simple science with children aged 5-10yrs and their parents/carers Favourable comments from the audience on how the stand sparked their interest. |
Year(s) Of Engagement Activity | 2014,2015 |
URL | http://rhizosphere.org/lab-news/ |
Description | Wymondham academy school visit |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Talk to 6th form students to encourage them to consider science as a career Students keen to apply for university places |
Year(s) Of Engagement Activity | 2014 |