The CHELL : A Bottom-Up approach to in vitro and in silico Minimal Life-like Constructs
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
Errey JC
(2010)
Mechanistic insight into enzymatic glycosyl transfer with retention of configuration through analysis of glycomimetic inhibitors.
in Angewandte Chemie (International ed. in English)
Fernández-González M
(2010)
Site-selective chemoenzymatic construction of synthetic glycoproteins using endoglycosidases
in Chemical Science
Floyd N
(2009)
Thiyl glycosylation of olefinic proteins: S-linked glycoconjugate synthesis.
in Angewandte Chemie (International ed. in English)
Floyd N
(2009)
Photoinduced, Family-Specific, Site-Selective Cleavage of TIM-Barrel Proteins
in Journal of the American Chemical Society
Floyd Nicola
(2009)
ORGN 260-An unexpected photo-induced site-selective protein cleavage reaction
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
French AC
(2009)
High-purity discrete PEG-oligomer crystals allow structural insight.
in Angewandte Chemie (International ed. in English)
Fulton Z.
(2009)
Molecular mechanisms for enhancing the antigenicity of the carbohydrate epitope of the broadly neutralizing anti-HIV-1 antibody 2G12
in RETROVIROLOGY
Gamblin DP
(2009)
Glycoprotein synthesis: an update.
in Chemical reviews
Gao Z
(2013)
Enhanced aqueous Suzuki-Miyaura coupling allows site-specific polypeptide 18F-labeling.
in Journal of the American Chemical Society
Gardner PM
(2009)
Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria.
in Nature chemistry
Garnett JA
(2009)
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
in Protein science : a publication of the Protein Society
Garnier P
(2010)
Lectin-directed enzyme activated prodrug therapy (LEAPT): Synthesis and evaluation of rhamnose-capped prodrugs.
in Journal of drug targeting
Goodfellow JJ
(2012)
An endoglycosidase with alternative glycan specificity allows broadened glycoprotein remodelling.
in Journal of the American Chemical Society
Grayson EJ
(2011)
A coordinated synthesis and conjugation strategy for the preparation of homogeneous glycoconjugate vaccine candidates.
in Angewandte Chemie (International ed. in English)
Guimaraes Andreia M. R.
(2011)
Glycosylation enhances selectivity of novobiocin and alters antibacterial mechanism
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Gunnoo SB
(2014)
Creation of a gated antibody as a conditionally functional synthetic protein.
in Nature communications
Harris LG
(2009)
Rewritable glycochips.
in Journal of the American Chemical Society
Hong S
(2010)
Synthesis and characterization of WS2 inorganic nanotubes with encapsulated/intercalated CsI
in Nano Research
Hong Sung You
(2009)
Functionalization of single walled carbon nanotubes with carbohydrates
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Hong SY
(2010)
Filled and glycosylated carbon nanotubes for in vivo radioemitter localization and imaging.
in Nature materials
Howden AJ
(2013)
QuaNCAT: quantitating proteome dynamics in primary cells.
in Nature methods
Kemper S
(2010)
Group epitope mapping considering relaxation of the ligand (GEM-CRL): including longitudinal relaxation rates in the analysis of saturation transfer difference (STD) experiments.
in Journal of magnetic resonance (San Diego, Calif. : 1997)
Kong L
(2013)
Single-molecule interrogation of a bacterial sugar transporter allows the discovery of an extracellular inhibitor.
in Nature chemistry
Kreizman R
(2009)
Core-shell PbI2@WS2 inorganic nanotubes from capillary wetting.
in Angewandte Chemie (International ed. in English)
Lee Seung Seo
(2011)
Biosynthesis of nucleoside antibiotic tunicamycin proceeds via a unique
exo-glycal intermediate
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Lee SS
(2011)
Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase.
in Nature chemical biology
Lercher L
(2013)
DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
in Angewandte Chemie (International ed. in English)
Lercher L
(2015)
Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation.
in Nature communications
Li WW
(2011)
Tuning the cavity of cyclodextrins: altered sugar adaptors in protein pores.
in Journal of the American Chemical Society
Lin Y
(2010)
The allylic chalcogen effect in olefin metathesis
in Beilstein Journal of Organic Chemistry
Lin YA
(2010)
Olefin cross-metathesis on proteins: investigation of allylic chalcogen effects and guiding principles in metathesis partner selection.
in Journal of the American Chemical Society
Lin YA
(2013)
Rapid cross-metathesis for reversible protein modifications via chemical access to Se-allyl-selenocysteine in proteins.
in Journal of the American Chemical Society
Lin YA
(2009)
Olefin metathesis for site-selective protein modification.
in Chembiochem : a European journal of chemical biology
Liu F
(2014)
Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.
in Journal of the American Chemical Society
Lo Conte M
(2011)
Multi-molecule reaction of serum albumin can occur through thiol-yne coupling.
in Chemical communications (Cambridge, England)
Mayorkas N
(2011)
Carbohydrate hydration: heavy water complexes of a and ß anomers of glucose, galactose, fucose and xylose.
in Physical chemistry chemical physics : PCCP
Mayorkas N
(2011)
Heavy water hydration of mannose: the anomeric effect in solvation, laid bare
in Chemical Science
Monrad R
(2014)
Dissecting the reaction of Phase II metabolites of ibuprofen and other NSAIDS with human plasma protein
in Chem. Sci.
Nielsen MM
(2011)
Substrate and metal ion promiscuity in mannosylglycerate synthase.
in The Journal of biological chemistry
Nunes C
(2013)
Inhibition of SnRK1 by metabolites: tissue-dependent effects and cooperative inhibition by glucose 1-phosphate in combination with trehalose 6-phosphate.
in Plant physiology and biochemistry : PPB
Parry AL
(2013)
'Multicopy multivalent' glycopolymer-stabilized gold nanoparticles as potential synthetic cancer vaccines.
in Journal of the American Chemical Society
Parry Alison L.
(2009)
Glycopolymer-functionalized gold nanoparticles: A new strategy toward synthetic anticancer vaccines
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Pasparakis G
(2010)
Controlled polymer synthesis--from biomimicry towards synthetic biology.
in Chemical Society reviews
Patel MK
(2013)
Control of phosphoryl migratory transesterifications allows regioselecive access to sugar phosphates.
in Organic letters
Patel MK
(2010)
Analysis of the dispersity in carbohydrate loading of synthetic glycoproteins using MALDI-TOF mass spectrometry.
in Chemical communications (Cambridge, England)
Patel MK
(2010)
Flow chemistry kinetic studies reveal reaction conditions for ready access to unsymmetrical trehalose analogues.
in Organic & biomolecular chemistry
Patel SM
(2011)
High throughput discovery of heteroaromatic-modifying enzymes allows enhancement of novobiocin selectivity.
in Chemical communications (Cambridge, England)
Pincu M
(2011)
Isotopic hydration of cellobiose: vibrational spectroscopy and dynamical simulations.
in The journal of physical chemistry. A
Pérez-Victoria I
(2009)
Saturation transfer difference NMR reveals functionally essential kinetic differences for a sugar-binding repressor protein.
in Chemical communications (Cambridge, England)
Description | We found that we were not only able to make an artificial chemical cell (something we called the 'chell') but that we could also create sufficient information from its metabolism for it to communicate with living (bacterial cells). This 'conversation' between artificial cells and living cells highlights that our notions of cellularity (and life) can be examined through fundamental experiments. |
Exploitation Route | These ideas have provoked various communities that examine cells right down to those who are interested in the emergence of complexity and even studying the origins of life. It provoked an analysis of the use of 'chemical cells' as potential 'living drugs', for example - see https://vimeo.com/10274649. |
Sectors | Education,Pharmaceuticals and Medical Biotechnology |
URL | http://users.ox.ac.uk/~dplb0149/index.html |
Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), newspapers (Times, New Scientist) science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. This work has been widely featured as one of the very first SynBio grants and that led to an expansion of the UK's SynBio community. It has also provoked a community of those more broadly interested in these concepts, including designers and artists - see https://vimeo.com/10274649 or http://www.daisyginsberg.com/work/synthesis-exchange-laboratory |
First Year Of Impact | 2009 |
Sector | Chemicals,Creative Economy,Education,Pharmaceuticals and Medical Biotechnology,Other |
Impact Types | Cultural,Societal,Economic,Policy & public services |
Company Name | Glycoform Ltd |
Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |