Nanoparticle Cytometrics: a quantitative analysis of the toxic effect of nanoparticles
Lead Research Organisation:
University of Leeds
Department Name: Institute of Materials Research
Abstract
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Organisations
Publications
Hondow N
(2014)
The use of transmission electron microscopy in the quantification of nanoparticle dose
in Journal of Physics: Conference Series
Singh N
(2012)
The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles.
in Biomaterials
Hondow N
(2012)
TEM analysis of nanoparticle dispersions with application towards the quantification of in vitro cellular uptake
in Journal of Physics: Conference Series
Hondow N
(2011)
STEM mode in the SEM: a practical tool for nanotoxicology.
in Nanotoxicology
Hondow N
(2012)
STEM mode in the SEM for the analysis of cellular sections prepared by ultramicrotome sectioning
in Journal of Physics: Conference Series
Brown MR
(2015)
Statistical prediction of nanoparticle delivery: from culture media to cell.
in Nanotechnology
Manshian BB
(2013)
Single-walled carbon nanotubes: differential genotoxic potential associated with physico-chemical properties.
in Nanotoxicology
Hondow N
(2012)
Quantitative characterization of nanoparticle agglomeration within biological media
in Journal of Nanoparticle Research
Hondow N
(2016)
Quantifying the cellular uptake of semiconductor quantum dot nanoparticles by analytical electron microscopy.
in Journal of microscopy
Summers HD
(2013)
Quantification of nanoparticle dose and vesicular inheritance in proliferating cells.
in ACS nano
Description | Key findings 1) We have used an electron microscope to measure the dispersion of nano particles in liquids (by rapidly freezing the liquid with the nano particles trapped in the suspension). This has enabled measurement of nano particle clustering in challenging liquids such as cell culture media which contain other suspended solids. Using the method we can establish whether individual or groups of nano particles are delivered to cells for toxicity texting or cell uptake studies. 2) We have used electron microscopy to count the number of fluorescent nano particles taken up by cells. By combining this count with the fluorescence intensity measured from many cells in a population we can estimate the average number and range of nano particles taken up by each cell across a cell population. This measure of dose will help us to predict the potential efficacy of a nano particle based medicine. 3) We have used electron microscopy to confirm that nano particles which fluoresce with different colours can be taken up separately by cells. This is a key step in a new method we have developed to accurately mark and track live cells over several hours. 4) We have assessed the toxic potential of semiconductor particles that fluoresce (and can be used as optical biomarkers) as a function of the surface charge on the particles. We suggest that negatively charge particles produce the most efficient uptake with lowest toxicity. |
Exploitation Route | Others will use the microscopy techniques developed in this work for similar nano toxicology and nano medicine investigations. |
Sectors | Healthcare Pharmaceuticals and Medical Biotechnology |
Description | So far our findings have been communicated by publication, at academic conferences (including for invited talks) and by presentation to regulatory bodies (the EU grant meetings) and external companies, such as AstraZeneca. We would expect other researchers to use the microscopy techniques developed in this work for similar nano toxicology and nano medicine investigations. The post-doctoral researcher employed on the contract has been awarded an independent research fellowship (AXA award and now a University Academic Fellowship). |
First Year Of Impact | 2011 |
Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Societal |