Nanoparticle Cytometrics: a quantitative analysis of the toxic effect of nanoparticles
Lead Research Organisation:
University of Leeds
Department Name: Institute of Materials Research
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
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Hondow N
(2014)
The use of transmission electron microscopy in the quantification of nanoparticle dose
in Journal of Physics: Conference Series
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Singh N
(2012)
The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles.
in Biomaterials
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Hondow N
(2012)
TEM analysis of nanoparticle dispersions with application towards the quantification of in vitro cellular uptake
in Journal of Physics: Conference Series
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Hondow N
(2011)
STEM mode in the SEM: a practical tool for nanotoxicology.
in Nanotoxicology
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Hondow N
(2012)
STEM mode in the SEM for the analysis of cellular sections prepared by ultramicrotome sectioning
in Journal of Physics: Conference Series
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Brown M
(2015)
Statistical prediction of nanoparticle delivery: from culture media to cell
in Nanotechnology
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Manshian BB
(2013)
Single-walled carbon nanotubes: differential genotoxic potential associated with physico-chemical properties.
in Nanotoxicology
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Hondow N
(2012)
Quantitative characterization of nanoparticle agglomeration within biological media
in Journal of Nanoparticle Research
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Hondow N
(2016)
Quantifying the cellular uptake of semiconductor quantum dot nanoparticles by analytical electron microscopy.
in Journal of microscopy
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Summers HD
(2013)
Quantification of nanoparticle dose and vesicular inheritance in proliferating cells.
in ACS nano
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Rees P
(2014)
Nanoparticle vesicle encoding for imaging and tracking cell populations.
in Nature methods
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Brown A
(2013)
Nanomedicine
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Brydson R
(2015)
Microscopy of nanoparticulate dispersions.
in Journal of microscopy
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Manshian BB
(2016)
Genotoxic capacity of Cd/Se semiconductor quantum dots with differing surface chemistries.
in Mutagenesis
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Booth M
(2012)
Determining the Concentration of CuInS 2 Quantum Dots from the Size-Dependent Molar Extinction Coefficient
in Chemistry of Materials
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Manshian BB
(2015)
Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.
in Toxicological sciences : an official journal of the Society of Toxicology
Description | Key findings 1) We have used an electron microscope to measure the dispersion of nano particles in liquids (by rapidly freezing the liquid with the nano particles trapped in the suspension). This has enabled measurement of nano particle clustering in challenging liquids such as cell culture media which contain other suspended solids. Using the method we can establish whether individual or groups of nano particles are delivered to cells for toxicity texting or cell uptake studies. 2) We have used electron microscopy to count the number of fluorescent nano particles taken up by cells. By combining this count with the fluorescence intensity measured from many cells in a population we can estimate the average number and range of nano particles taken up by each cell across a cell population. This measure of dose will help us to predict the potential efficacy of a nano particle based medicine. 3) We have used electron microscopy to confirm that nano particles which fluoresce with different colours can be taken up separately by cells. This is a key step in a new method we have developed to accurately mark and track live cells over several hours. 4) We have assessed the toxic potential of semiconductor particles that fluoresce (and can be used as optical biomarkers) as a function of the surface charge on the particles. We suggest that negatively charge particles produce the most efficient uptake with lowest toxicity. |
Exploitation Route | Others will use the microscopy techniques developed in this work for similar nano toxicology and nano medicine investigations. |
Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
Description | So far our findings have been communicated by publication, at academic conferences (including for invited talks) and by presentation to regulatory bodies (the EU grant meetings) and external companies, such as AstraZeneca. We would expect other researchers to use the microscopy techniques developed in this work for similar nano toxicology and nano medicine investigations. The post-doctoral researcher employed on the contract has been awarded an independent research fellowship (AXA award and now a University Academic Fellowship). |
First Year Of Impact | 2011 |
Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Societal |