Developing ways of implementing biocatalytic hydrogenation reactions in flow

Lead Research Organisation: University of Oxford
Department Name: Interdisciplinary Bioscience DTP

Abstract

Widely applicable approaches to using NADH-dependent enzymes for highly selective redox steps in fine chemical synthesis are still lacking. This project exploits a novel H2-driven method for NAD(P)H recycling in which all enzymes are immobilised on carbon beads; this systems overcomes challenges with atom efficient cofactor recycling and simple enzyme handling, recovery and reuse. For these reasons, this system is also uniquely placed for translating biocatalysis into flow reactors, and thus establishes a new way of working that will be of great value in the industrial biotechnology sector.
BBSRC priorities areas and aims
This project sits firmly within the priority area of developing a "new strategic approach to industrial biotechnology" because it develops a methodology that will be broadly applicable to support uptake of biocatalysis for chemical synthesis. IBB, ENWW

Publications

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Poznansky B (2020) Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow in Organic Process Research & Development

Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M011224/1 01/10/2015 30/09/2023
1946990 Studentship BB/M011224/1 01/10/2017 30/09/2021 Barnabas Jonthan Poznansky
 
Description A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L-1 h-1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.
Exploitation Route Spur on further research in the field.
Sectors Environment,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

URL https://pubs.acs.org/doi/10.1021/acs.oprd.9b00410
 
Description Dr Reddy's 
Organisation Dr. Reddy's Laboratories
Country India 
Sector Private 
PI Contribution I have spent a month at Dr Reddy's as part of my DPhil Project. I will spend a further 2 months there, this year.
Collaborator Contribution Dr Reddy's have provided lab space, equipment, and directional input on my project. They also provided some data for our joint publication.
Impact 1 publication listed in the publication section
Start Year 2017