Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Chooi K. Phin
(2014)
Giving p38a kinase a jump-start: p38a Activation induced by chemical modification
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chooi KP
(2014)
Synthetic phosphorylation of p38a recapitulates protein kinase activity.
in Journal of the American Chemical Society
Cobo I
(2012)
Phosphine-free Suzuki-Miyaura cross-coupling in aqueous media enables access to 2-C-aryl-glycosides.
in Organic letters
Cocinero EJ
(2011)
Sensing the anomeric effect in a solvent-free environment.
in Nature
Cocinero EJ
(2013)
Free fructose is conformationally locked.
in Journal of the American Chemical Society
Cocinero EJ
(2011)
Exploring carbohydrate-peptide interactions in the gas phase: structure and selectivity in complexes of pyranosides with N-acetylphenylalanine methylamide.
in Journal of the American Chemical Society
Cruz IN
(2013)
Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.
in Chemical science
Dadová J
(2017)
Precise Probing of Residue Roles by Post-Translational ß,?-C,N Aza-Michael Mutagenesis in Enzyme Active Sites.
in ACS central science
Davis Ben
(2012)
Sugars and proteins
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
De Munari S
(2015)
A Triply Divergent Reagent for Glycoprotein Synthesis
in Israel Journal of Chemistry
Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
URL | http://users.ox.ac.uk/~dplb0149/ |
Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
First Year Of Impact | 2011 |
Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural,Societal,Economic |
Company Name | Glycoform Ltd |
Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |