Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Dumas A
(2015)
Designing logical codon reassignment - Expanding the chemistry in biology.
in Chemical science
Dumas A
(2013)
Self-liganded Suzuki-Miyaura coupling for site-selective protein PEGylation.
in Angewandte Chemie (International ed. in English)
Díaz-Rodríguez A
(2011)
Chemical modification in the creation of novel biocatalysts.
in Current opinion in chemical biology
Gao Z
(2013)
Enhanced aqueous Suzuki-Miyaura coupling allows site-specific polypeptide 18F-labeling.
in Journal of the American Chemical Society
Goodfellow JJ
(2012)
An endoglycosidase with alternative glycan specificity allows broadened glycoprotein remodelling.
in Journal of the American Chemical Society
Grayson EJ
(2011)
A coordinated synthesis and conjugation strategy for the preparation of homogeneous glycoconjugate vaccine candidates.
in Angewandte Chemie (International ed. in English)
Guimaraes Andreia M. R.
(2011)
Glycosylation enhances selectivity of novobiocin and alters antibacterial mechanism
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Gunnoo SB
(2014)
Creation of a gated antibody as a conditionally functional synthetic protein.
in Nature communications
Howden AJ
(2013)
QuaNCAT: quantitating proteome dynamics in primary cells.
in Nature methods
Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
URL | http://users.ox.ac.uk/~dplb0149/ |
Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
First Year Of Impact | 2011 |
Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural,Societal,Economic |
Company Name | Glycoform Ltd |
Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |