Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Mayorkas N
(2011)
Carbohydrate hydration: heavy water complexes of a and ß anomers of glucose, galactose, fucose and xylose.
in Physical chemistry chemical physics : PCCP
Monrad R
(2014)
Dissecting the reaction of Phase II metabolites of ibuprofen and other NSAIDS with human plasma protein
in Chem. Sci.
Nielsen MM
(2011)
Substrate and metal ion promiscuity in mannosylglycerate synthase.
in The Journal of biological chemistry
Nunes C
(2013)
Inhibition of SnRK1 by metabolites: tissue-dependent effects and cooperative inhibition by glucose 1-phosphate in combination with trehalose 6-phosphate.
in Plant physiology and biochemistry : PPB
Parry AL
(2013)
'Multicopy multivalent' glycopolymer-stabilized gold nanoparticles as potential synthetic cancer vaccines.
in Journal of the American Chemical Society
Patel MK
(2013)
Control of phosphoryl migratory transesterifications allows regioselecive access to sugar phosphates.
in Organic letters
Patel SM
(2011)
High throughput discovery of heteroaromatic-modifying enzymes allows enhancement of novobiocin selectivity.
in Chemical communications (Cambridge, England)
Pincu M
(2011)
Isotopic hydration of cellobiose: vibrational spectroscopy and dynamical simulations.
in The journal of physical chemistry. A
Pérez-Victoria I
(2015)
Glycosyldiselenides as lectin ligands detectable by NMR in biofluids.
in Chemical communications (Cambridge, England)
Ribeiro-Viana R
(2012)
Virus-like glycodendrinanoparticles displaying quasi-equivalent nested polyvalency upon glycoprotein platforms potently block viral infection.
in Nature communications
Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
URL | http://users.ox.ac.uk/~dplb0149/ |
Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
First Year Of Impact | 2011 |
Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural,Societal,Economic |
Company Name | Glycoform Ltd |
Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |