Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Kong L
(2013)
Single-molecule interrogation of a bacterial sugar transporter allows the discovery of an extracellular inhibitor.
in Nature chemistry
Lee Seung Seo
(2011)
Biosynthesis of nucleoside antibiotic tunicamycin proceeds via a unique
exo-glycal intermediate
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Lee SS
(2011)
Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase.
in Nature chemical biology
Lercher L
(2015)
Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation.
in Nature communications
Lercher L
(2013)
DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
in Angewandte Chemie (International ed. in English)
Li WW
(2011)
Tuning the cavity of cyclodextrins: altered sugar adaptors in protein pores.
in Journal of the American Chemical Society
Lin YA
(2013)
Rapid cross-metathesis for reversible protein modifications via chemical access to Se-allyl-selenocysteine in proteins.
in Journal of the American Chemical Society
Liu F
(2014)
Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.
in Journal of the American Chemical Society
Lo Conte M
(2011)
Multi-molecule reaction of serum albumin can occur through thiol-yne coupling.
in Chemical communications (Cambridge, England)
Mayorkas N
(2011)
Heavy water hydration of mannose: the anomeric effect in solvation, laid bare
in Chemical Science
Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
URL | http://users.ox.ac.uk/~dplb0149/ |
Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
First Year Of Impact | 2011 |
Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural,Societal,Economic |
Company Name | Glycoform Ltd |
Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |